| Detection of Aβ plaques by a novel specific MRI probe precursor CR-BSA-(Gd-DTPA)n in APP/PS1 transgenic mice. | |
| | |
MedLine Citation:
|
PMID: 21089051 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Amyloid plaques are one of the hallmarks of Alzheimer's disease (AD). The lack of specific probes that can detect individual senile plaques in AD has prompted the development of magnetic resonance imaging (MRI) probes. In this study, based on DTPA-gadolinium (III) and congo red (CR), a novel specific MRI probe precursor CR-BSA-(Gd-DTPA)n was successfully synthesized. Its ability to bind to amyloid plaques was evaluated by brain sections from APP/PS1 transgenic mice. Its specificity for Aβ plaques was further demonstrated by immunohistochemistry (IHC) staining with the monoclonal antibody to the Aβ protein. Meanwhile, the amyloid deposits detected by the CR-BSA-(Gd-DTPA)n were matched to the amyloid deposits detected by Aβ specific antibody. We also found that a few amyloid-like deposits which was not detected by IHC. The findings indicated that the probe perhaps could detect the neurofibrillary tangles (NFT) similar to the effect of CR itself, and this will be verified in future experiments. The works suggested that the Aβ protein-specific magnetic resonance contrast agent precursor CR-BSA-(Gd-DTPA)n can be used as a potential fluorescence and MR multi-modal imaging probe precursor to display individual senile plaques in AD. |
| | |
Authors:
|
Shuping Li; Huijin He; Weigang Cui; Bing Gu; Jin Li; Zengxin Qi; Guomin Zhou; Chun-Min Liang; Xiao-Yuan Feng |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Anatomical record (Hoboken, N.J. : 2007) Volume: 293 ISSN: 1932-8494 ISO Abbreviation: Anat Rec (Hoboken) Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101292775 Medline TA: Anat Rec (Hoboken) Country: United States |
Other Details:
|
Languages: eng Pagination: 2136-43 Citation Subset: IM |
Affiliation:
|
Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Microsurgical anatomy of lumbosacral nerve rootlets for highly selective rhizotomy in chronic spinal...
Next Document: Expression of matrix metalloproteinase-9 mRNA and vascular endothelial growth factor protein in gast...