| Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | |
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MedLine Citation:
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PMID: 22553235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a potentially useful tool for the detection of antimicrobial resistance, especially that conferred by β-lactamases. Here we describe a modification of a previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing members of the Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii isolates, and 35 carbapenem-resistant enterobacteria producing no carbapenemase. The detection of carbapenemases by MALDI-TOF MS seems to be a powerful, quick, and cost-effective method for microbiological laboratories. |
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Authors:
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Jaroslav Hrabák; Vendula Studentová; Radka Walková; Helena Zemlicková; Vladislav Jakubu; Eva Chudácková; Marek Gniadkowski; Yvonne Pfeifer; John D Perry; Kathryn Wilkinson; Tamara Bergerová |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2012-05-02 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 50 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-19 Completed Date: 2012-10-17 Revised Date: 2013-04-15 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2441-3 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic. jaroslav.hrabak@lfp.cuni.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter baumannii
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enzymology* Bacterial Proteins / metabolism* Enterobacteriaceae / enzymology* Enterobacteriaceae Infections / microbiology Humans Hydrolysis Microbial Sensitivity Tests / methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Thienamycins / metabolism* beta-Lactamases / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Thienamycins; 96036-03-2/meropenem; EC 3.5.2.6/beta-Lactamases; EC 3.5.2.6/carbapenemase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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