Document Detail


Detection of N-terminal pro-corticotrophin releasing hormone (CRH) and a 'novel' CRH in human maternal plasma and placenta.
MedLine Citation:
PMID:  10736245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Corticotrophin-releasing hormone (CRH) is released from the human placenta in high concentrations towards the end of the third trimester of pregnancy, but relative concentrations of other cleavage products derived from the CRH prohormone are unknown. We have measured CRH and N-terminal (1-100) and (1-127) proCRH peptides in maternal plasma in the second and third trimesters of pregnancy and in term placental extract using immunoradiometric assays (IRMAs) specific to different regions of the CRH precursor. Levels of N-terminal proCRH (amino acid residues 1-100) rose from 24+/-4 pmol/l (mean+/-s.e.) in the second trimester to 378.8+/-65 pmol/l (mean+/-s.e.) at term. Levels of intact proCRH and/or (1-127) proCRH remained relatively constant throughout the second and third trimesters, with a concentration of 29.3+/-3.8 pmol/l (mean+/-s.e.). In the course of this work a novel form of CRH that cross-reacts within specific CRH immunoassays was observed. The use of two IRMAs developed for CRH (1-41) having different C-terminal epitope specificities provided evidence for two types of CRH coexisting in maternal plasma. Separation of term placental extract by HPLC and application of the two CRH IRMAs revealed two peaks of immunoreactivity one of which coeluted with synthetic CRH (1-41).
Authors:
T C Sanderson; R J Woods; C F Kemp; P J Lowry
Related Documents :
1450415 - Human macrophage colony-stimulating factor levels are elevated in pregnancy and in immu...
1053545 - A combined ultrasonic and biochemical study of fetal renal function in the term fetus.
6894035 - Prostaglandins in the human fetal circulation in mid-trimester and term pregnancy.
24612935 - A rare case of rapidly enlarging tracheal lobular capillary hemangioma presenting as di...
8600265 - Considerations in the management of maxillofacial infections in the pregnant patient.
7240475 - Pruritic urticarial papules and plaques of pregnancy.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Placenta     Volume:  21     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    2000 Mar-Apr
Date Detail:
Created Date:  2000-05-25     Completed Date:  2000-05-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  218-25     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Harcourt Publishers Ltd.
Affiliation:
School of Animal and Microbial Sciences, University of Reading, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Corticotropin-Releasing Hormone / blood*,  immunology,  metabolism*
Cross Reactions
Female
Humans
Immunoradiometric Assay
Peptide Fragments / blood*,  immunology,  metabolism*
Placenta / metabolism*
Pregnancy / blood*,  metabolism*
Protein Precursors / blood*,  immunology,  metabolism*
Chemical
Reg. No./Substance:
0/Peptide Fragments; 0/Protein Precursors; 0/pro-corticotropin releasing hormone; 9015-71-8/Corticotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differential regulation in human amnion epithelial and fibroblast cells of prostaglandin E(2) produc...
Next Document:  Immunohistochemical localization of ECE-1 in the human placenta.