| Detection of N-terminal pro-corticotrophin releasing hormone (CRH) and a 'novel' CRH in human maternal plasma and placenta. | |
| | |
MedLine Citation:
|
PMID: 10736245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Corticotrophin-releasing hormone (CRH) is released from the human placenta in high concentrations towards the end of the third trimester of pregnancy, but relative concentrations of other cleavage products derived from the CRH prohormone are unknown. We have measured CRH and N-terminal (1-100) and (1-127) proCRH peptides in maternal plasma in the second and third trimesters of pregnancy and in term placental extract using immunoradiometric assays (IRMAs) specific to different regions of the CRH precursor. Levels of N-terminal proCRH (amino acid residues 1-100) rose from 24+/-4 pmol/l (mean+/-s.e.) in the second trimester to 378.8+/-65 pmol/l (mean+/-s.e.) at term. Levels of intact proCRH and/or (1-127) proCRH remained relatively constant throughout the second and third trimesters, with a concentration of 29.3+/-3.8 pmol/l (mean+/-s.e.). In the course of this work a novel form of CRH that cross-reacts within specific CRH immunoassays was observed. The use of two IRMAs developed for CRH (1-41) having different C-terminal epitope specificities provided evidence for two types of CRH coexisting in maternal plasma. Separation of term placental extract by HPLC and application of the two CRH IRMAs revealed two peaks of immunoreactivity one of which coeluted with synthetic CRH (1-41). |
| | |
Authors:
|
T C Sanderson; R J Woods; C F Kemp; P J Lowry |
Related Documents
:
|
1450415 - Human macrophage colony-stimulating factor levels are elevated in pregnancy and in immu... 1053545 - A combined ultrasonic and biochemical study of fetal renal function in the term fetus. 6894035 - Prostaglandins in the human fetal circulation in mid-trimester and term pregnancy. 1725365 - Comparison of endothelin-1 concentrations in normal and complicated pregnancies. 8600265 - Considerations in the management of maxillofacial infections in the pregnant patient. 7240475 - Pruritic urticarial papules and plaques of pregnancy. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Placenta Volume: 21 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2000 Mar-Apr |
Date Detail:
|
Created Date: 2000-05-25 Completed Date: 2000-05-25 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8006349 Medline TA: Placenta Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 218-25 Citation Subset: IM |
Copyright Information:
|
Copyright 2000 Harcourt Publishers Ltd. |
Affiliation:
|
School of Animal and Microbial Sciences, University of Reading, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Corticotropin-Releasing Hormone
/
blood*,
immunology,
metabolism* Cross Reactions Female Humans Immunoradiometric Assay Peptide Fragments / blood*, immunology, metabolism* Placenta / metabolism* Pregnancy / blood*, metabolism* Protein Precursors / blood*, immunology, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Peptide Fragments; 0/Protein Precursors; 0/pro-corticotropin releasing hormone; 9015-71-8/Corticotropin-Releasing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differential regulation in human amnion epithelial and fibroblast cells of prostaglandin E(2) produc...
Next Document: Immunohistochemical localization of ECE-1 in the human placenta.