Document Detail


Detection of myocardial ischemia-reperfusion injury using a fluorescent near-infrared zinc(II)-dipicolylamine probe and 99mTc glucarate.
MedLine Citation:
PMID:  22554483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fluorescent zinc 2,2'-dipicolylamine coordination complex PSVue®794 (probe 1) is known to selectively bind to phosphatidylserine exposed on the surface of apoptotic and necrotic cells. In this study, we investigated the cell death targeting properties of probe 1 in myocardial ischemia-reperfusion injury. A rat heart model of ischemia-reperfusion was used. Probe 1, control dye, or 99mTc glucarate was intravenously injected in rats subjected to 30-minute and 5-minute myocardial ischemia followed by 2-hour reperfusion. At 90 minutes or 20 hours postinjection, myocardial uptake was evaluated ex vivo by fluorescence imaging and autoradiography. Hematoxylin-eosin and cleaved caspase-3 staining was performed on myocardial sections to demonstrate the presence of ischemia-reperfusion injury and apoptosis. Selective accumulation of probe 1 could be detected in the area at risk up to 20 hours postinjection. Similar topography and extent of uptake of probe 1 and 99mTc glucarate were observed at 90 minutes postinjection. Histologic analysis demonstrated the presence of necrosis, but only a few apoptotic cells could be detected. Probe 1 selectively accumulates in myocardial ischemia-reperfusion injury and is a promising cell death imaging tool.
Authors:
Leonie Wyffels; Brian D Gray; Christy Barber; Koon Y Pak; Safiyyah Forbes; Jeffrey A Mattis; James M Woolfenden; Zhonglin Liu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular imaging     Volume:  11     ISSN:  1536-0121     ISO Abbreviation:  Mol Imaging     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-04     Completed Date:  2012-09-10     Revised Date:  2013-12-06    
Medline Journal Info:
Nlm Unique ID:  101120118     Medline TA:  Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amines / chemistry*
Animals
Fluorescent Dyes / diagnostic use*
Glucaric Acid / analogs & derivatives*,  diagnostic use
Immunohistochemistry
Male
Myocardial Reperfusion Injury / diagnosis*
Organotechnetium Compounds / diagnostic use*
Picolinic Acids / chemistry*
Radiopharmaceuticals / diagnostic use*
Rats
Rats, Sprague-Dawley
Zinc / chemistry*
Grant Support
ID/Acronym/Agency:
P41-EB002035/EB/NIBIB NIH HHS; R01 HL090716/HL/NHLBI NIH HHS; R01-HL090716/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/2,2'-dipicolylamine; 0/Amines; 0/Fluorescent Dyes; 0/Organotechnetium Compounds; 0/Picolinic Acids; 0/Radiopharmaceuticals; 0/technetium Tc 99m glucarate; J41CSQ7QDS/Zinc; QLZ991V4A2/Glucaric Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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