Document Detail


Detection of Mycobacterium tuberculosis in cerebrospinal fluid following immunomagnetic enrichment.
MedLine Citation:
PMID:  8789037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The detection of Mycobacterium tuberculosis by culture of cerebrospinal fluid (CSF) is unacceptably slow. Low numbers of organisms and the presence of reaction inhibitors may prevent detection of M. tuberculosis by PCR. We used immunomagnetic enrichment to accelerate and enhance the detection of mycobacteria in CSF after demonstrating the utility of the method with pure suspensions. Growth was detected earlier in Bactec cultures of magnetically recovered mycobacteria than in untreated CSF (7 versus 15 days). We detected M. tuberculosis DNA by PCR in the immunomagnetically enriched sample but not in untreated CSF. PCR fingerprintings of the immunomagnetically recovered M. tuberculosis and of the isolate subsequently recovered by culture were identical.
Authors:
G H Mazurek; V Reddy; D Murphy; T Ansari
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  34     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-10-21     Completed Date:  1996-10-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  450-3     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Texas Health Center at Tyler.
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MeSH Terms
Descriptor/Qualifier:
Adult
Bacteriological Techniques*
Cerebrospinal Fluid / microbiology*
DNA Fingerprinting
DNA, Bacterial / cerebrospinal fluid,  genetics
Evaluation Studies as Topic
Female
Humans
Immunomagnetic Separation / methods*
Mycobacterium tuberculosis / genetics,  growth & development,  isolation & purification*
Polymerase Chain Reaction
Time Factors
Tuberculosis, Meningeal / cerebrospinal fluid,  diagnosis*,  microbiology
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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