| Detection of Mycobacterium tuberculosis in cerebrospinal fluid following immunomagnetic enrichment. | |
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MedLine Citation:
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PMID: 8789037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The detection of Mycobacterium tuberculosis by culture of cerebrospinal fluid (CSF) is unacceptably slow. Low numbers of organisms and the presence of reaction inhibitors may prevent detection of M. tuberculosis by PCR. We used immunomagnetic enrichment to accelerate and enhance the detection of mycobacteria in CSF after demonstrating the utility of the method with pure suspensions. Growth was detected earlier in Bactec cultures of magnetically recovered mycobacteria than in untreated CSF (7 versus 15 days). We detected M. tuberculosis DNA by PCR in the immunomagnetically enriched sample but not in untreated CSF. PCR fingerprintings of the immunomagnetically recovered M. tuberculosis and of the isolate subsequently recovered by culture were identical. |
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Authors:
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G H Mazurek; V Reddy; D Murphy; T Ansari |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 34 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-10-21 Completed Date: 1996-10-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 450-3 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Texas Health Center at Tyler. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bacteriological Techniques* Cerebrospinal Fluid / microbiology* DNA Fingerprinting DNA, Bacterial / cerebrospinal fluid, genetics Evaluation Studies as Topic Female Humans Immunomagnetic Separation / methods* Mycobacterium tuberculosis / genetics, growth & development, isolation & purification* Polymerase Chain Reaction Time Factors Tuberculosis, Meningeal / cerebrospinal fluid, diagnosis*, microbiology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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