Document Detail

Detection of Mycobacterium tuberculosis in cerebrospinal fluid following immunomagnetic enrichment.
MedLine Citation:
PMID:  8789037     Owner:  NLM     Status:  MEDLINE    
The detection of Mycobacterium tuberculosis by culture of cerebrospinal fluid (CSF) is unacceptably slow. Low numbers of organisms and the presence of reaction inhibitors may prevent detection of M. tuberculosis by PCR. We used immunomagnetic enrichment to accelerate and enhance the detection of mycobacteria in CSF after demonstrating the utility of the method with pure suspensions. Growth was detected earlier in Bactec cultures of magnetically recovered mycobacteria than in untreated CSF (7 versus 15 days). We detected M. tuberculosis DNA by PCR in the immunomagnetically enriched sample but not in untreated CSF. PCR fingerprintings of the immunomagnetically recovered M. tuberculosis and of the isolate subsequently recovered by culture were identical.
G H Mazurek; V Reddy; D Murphy; T Ansari
Related Documents :
23765607 - Effect of laminated hydroxyapatite/gelatin nanocomposite scaffold structure on osteogen...
23495227 - Large-scale expansion of pre-isolated bone marrow mesenchymal stromal cells in serum-fr...
17297247 - The interval between initiation of anti-tuberculosis treatment in patients with culture...
23028757 - Aging effect on neurotrophic activity of human mesenchymal stem cells.
8442437 - Leukemia inhibitory factor inhibits osteoclastic resorption, growth, mineralization, an...
11165347 - The use of a solid adsorber resin for enrichment of bacteria with toxic substrates and ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  34     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-10-21     Completed Date:  1996-10-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  450-3     Citation Subset:  IM    
Department of Medicine, University of Texas Health Center at Tyler.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacteriological Techniques*
Cerebrospinal Fluid / microbiology*
DNA Fingerprinting
DNA, Bacterial / cerebrospinal fluid,  genetics
Evaluation Studies as Topic
Immunomagnetic Separation / methods*
Mycobacterium tuberculosis / genetics,  growth & development,  isolation & purification*
Polymerase Chain Reaction
Time Factors
Tuberculosis, Meningeal / cerebrospinal fluid,  diagnosis*,  microbiology
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Epidemiological study of Mycoplasma pneumoniae infections in japan based on PCR-restriction fragment...
Next Document:  Direct identification and recognition of yeast species from clinical material by using albicans ID a...