Document Detail


Detection of Mycobacterium tuberculosis from sputum collected on filter paper and stored at room temperature for 5 days by PCR assay and culture.
MedLine Citation:
PMID:  11758856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONCLUSION: The collection and storage of sputum on filter paper at room temperature for 5 days had no apparent effect on the performance of nested PCR. Sputum samples collected by this method could be sent by post in a minimum of space and inexpensive way and will enable a large number of samples collected in the field or from peripheral health centers to be sent to central laboratories for analysis by trained technicians and under a well-equipped and well-established quality control system. The rapid and reliable detection by PCR-based assay will be helpful for optimal patient management of therapy and effective control of tuberculosis.
Authors:
U Tansuphasiri
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of the Medical Association of Thailand = Chotmaihet thangphaet     Volume:  84     ISSN:  0125-2208     ISO Abbreviation:  J Med Assoc Thai     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-12-07     Completed Date:  2002-01-14     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  7507216     Medline TA:  J Med Assoc Thai     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  1183-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques / instrumentation*,  methods*,  standards
Clinical Laboratory Techniques / instrumentation,  standards
DNA, Bacterial / analysis*
Filtration / instrumentation*,  methods*
Fluorescent Dyes / diagnostic use
Humans
Mycobacterium tuberculosis / genetics*,  isolation & purification*
Polymerase Chain Reaction / instrumentation*,  methods*,  standards
Quality Control
Sensitivity and Specificity
Specimen Handling / instrumentation*,  methods*,  standards
Sputum / microbiology*
Temperature*
Thailand
Time Factors
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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