| Detection of Mycobacterium tuberculosis from sputum collected on filter paper and stored at room temperature for 5 days by PCR assay and culture. | |
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MedLine Citation:
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PMID: 11758856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CONCLUSION: The collection and storage of sputum on filter paper at room temperature for 5 days had no apparent effect on the performance of nested PCR. Sputum samples collected by this method could be sent by post in a minimum of space and inexpensive way and will enable a large number of samples collected in the field or from peripheral health centers to be sent to central laboratories for analysis by trained technicians and under a well-equipped and well-established quality control system. The rapid and reliable detection by PCR-based assay will be helpful for optimal patient management of therapy and effective control of tuberculosis. |
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Authors:
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U Tansuphasiri |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Journal of the Medical Association of Thailand = Chotmaihet thangphaet Volume: 84 ISSN: 0125-2208 ISO Abbreviation: J Med Assoc Thai Publication Date: 2001 Aug |
Date Detail:
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Created Date: 2001-12-07 Completed Date: 2002-01-14 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 7507216 Medline TA: J Med Assoc Thai Country: Thailand |
Other Details:
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Languages: eng Pagination: 1183-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques
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instrumentation*,
methods*,
standards Clinical Laboratory Techniques / instrumentation, standards DNA, Bacterial / analysis* Filtration / instrumentation*, methods* Fluorescent Dyes / diagnostic use Humans Mycobacterium tuberculosis / genetics*, isolation & purification* Polymerase Chain Reaction / instrumentation*, methods*, standards Quality Control Sensitivity and Specificity Specimen Handling / instrumentation*, methods*, standards Sputum / microbiology* Temperature* Thailand Time Factors |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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