| Detection of Mycobacterium tuberculosis complex and Mycobacterium gordonae on the same portable surface plasmon resonance sensor. | |
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MedLine Citation:
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PMID: 20667437 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In the present study, we have developed a specific detection system for Mycobacterium tuberculosis complex (MTB complex) and Mycobacterium gordonae by using a commercially available SPR based portable-multichannel sensor system. The probe single-strand oligodeoxynucleotides (probe-ssODNs), which also contain suitable spacer arms, against the target characteristic sequence (target-ssODNs) of both species were selected and synthesized. The SPR sensors were prepared by direct coupling of thiolated probes on gold-coated sensor surfaces. 6-mercapto-1-hexanol was used as orientation helper and surface blocking agent. Immobilization protocol was optimized. The validation test results showed that the detection limit of sensor platform has been found to be 30 ng μl(-1). Developed sensor can be used to detect specific DNA hybridization at a concentration of 0.05 μM. The sensor chip surface can be regenerated with exposure to 2.5 mM HCl quite effectively and reused several times without losing the signal intensity. The SPR sensors carrying the probe-ssODNs were kept in vacuum at room temperature in the dark for about 12 weeks, and used effectively. |
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Authors:
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Memed Duman; Erhan Piskin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-08 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 26 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 908-12 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Hacettepe University, Chemical Engineering Department and Bioengineering Division, and R&D Center for Bioengineering and Biyomedtek, 06800 Ankara, Turkey. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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