| Detection of Mycobacterium tuberculosis antigens in urinary proteins of tuberculosis patients. | |
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MedLine Citation:
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PMID: 11913494 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) that can detect up to 0.5 ng of culture filtrate proteins (CFPs) of Mycobacterium tuberculosis strain H37Ra is described. This detection system features several special characteristics: (i) the use of CFPs from the H37Ra strain of Mycobacterium tuberculosis to generate capture and detection antibodies in rabbits and mice, respectively; (ii) affinity purification of CFP-reactive antibodies and selection of the antibody preparations for best performance in the sandwich ELISA system; and (iii) the use of urine-derived protein preparations for antigen detection. The sandwich ELISA could detect up to 0.5 ng of CFPs of the H37Ra strain of Mycobacterium tuberculosis. The assay did not detect antigens of Escherichia coli, Candida albicans or Saccharomyces cerivisiae but efficiently detected CFP preparations from nine different clinical isolates of Mycobacterium tuberculosis. Significant variations, however, were noted in the relative efficacy of the assay to detect CFPs from different clinical isolates of Mycobacterium tuberculosis. The procedure was utilized for detecting tubercular antigens in urine samples from 29 patients with confirmed (sputum-positive) tuberculosis and from 25 healthy controls. Significant levels of antigen could be detected in 22 of the 29 samples tested. |
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Authors:
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V Choudhry; R K Saxena |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology Volume: 21 ISSN: 0934-9723 ISO Abbreviation: Eur. J. Clin. Microbiol. Infect. Dis. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2002-03-26 Completed Date: 2002-09-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8804297 Medline TA: Eur J Clin Microbiol Infect Dis Country: Germany |
Other Details:
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Languages: eng Pagination: 1-5 Citation Subset: IM |
Affiliation:
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School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Bacterial / analysis, urine* Enzyme-Linked Immunosorbent Assay / methods* Humans Mice Mice, Inbred Strains Mycobacterium tuberculosis / immunology* Proteins / analysis Rabbits Reference Values Sensitivity and Specificity Tuberculosis / immunology*, urine Urinalysis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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