| Detection of Listeria monocytogenes in ready-to-eat food by Step One real-time polymerase chain reaction. | |
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MedLine Citation:
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PMID: 22375593 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The aim of this study was to follow contamination of ready-to-eat food with Listeria monocytogenes by using the Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. In 30 samples of ready-to-eat milk and meat products without incubation we detected strains of Listeria monocytogenes in five samples (swabs). Internal positive control (IPC) was positive in all samples. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in ready-to-eat food without incubation. |
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Authors:
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Jaroslav Pochop; Miroslava Kačániová; Lukáš Hleba; L'ubomír Lopasovský; Alica Bobková; Lucia Zeleňáková; Michal Stričík |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes Volume: 47 ISSN: 1532-4109 ISO Abbreviation: J Environ Sci Health B Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-03-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7607167 Medline TA: J Environ Sci Health B Country: England |
Other Details:
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Languages: eng Pagination: 212-6 Citation Subset: IM |
Affiliation:
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a Department of Microbiology , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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