Document Detail


Detection of Listeria monocytogenes in ready-to-eat food by Step One real-time polymerase chain reaction.
MedLine Citation:
PMID:  22375593     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The aim of this study was to follow contamination of ready-to-eat food with Listeria monocytogenes by using the Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. In 30 samples of ready-to-eat milk and meat products without incubation we detected strains of Listeria monocytogenes in five samples (swabs). Internal positive control (IPC) was positive in all samples. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in ready-to-eat food without incubation.
Authors:
Jaroslav Pochop; Miroslava Kačániová; Lukáš Hleba; L'ubomír Lopasovský; Alica Bobková; Lucia Zeleňáková; Michal Stričík
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes     Volume:  47     ISSN:  1532-4109     ISO Abbreviation:  J Environ Sci Health B     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7607167     Medline TA:  J Environ Sci Health B     Country:  England    
Other Details:
Languages:  eng     Pagination:  212-6     Citation Subset:  IM    
Affiliation:
a Department of Microbiology , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
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