Document Detail


Detection of Leptospira spp. in semen and vaginal fluids of goats and sheep by polymerase chain reaction.
MedLine Citation:
PMID:  18291518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thirteen goat herds and seven sheep flocks in the state of Rio de Janeiro, Brazil were screened for leptospirosis. From the three herds and three flocks with greatest seroreactivity, 19 goats (16 females and three bucks) and 40 sheep (26 ewes and 14 rams) that were seropositive (specific anti-Leptospira titres > or =400, based on a microscopic agglutination test), were selected for more detailed studies. From those animals, samples of vaginal fluids or semen were collected for bacteriological and molecular assays. For both species of animals, the most prevalent reactions were to serovars Hardjo, Shermani, and Grippotyphosa. Although leptospires were detected by darkfield microscopy in three vaginal fluid samples (from two goats and one ewe), pure isolates were not obtained by bacteriological culture of vaginal fluids or semen. However, seven vaginal fluid samples (from four goats and three ewes) and six semen samples (all from rams) were positive on polymerase chain reaction (PCR). Based on these findings, in addition to analogous findings in cattle, we inferred that there is potential for venereal transmission of leptospirosis in small ruminants.
Authors:
W Lilenbaum; R Varges; F Z Brandão; A Cortez; S O de Souza; P E Brandão; L J Richtzenhain; S A Vasconcellos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-04
Journal Detail:
Title:  Theriogenology     Volume:  69     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  837-42     Citation Subset:  IM    
Affiliation:
Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Universidade Federal Fluminense, 24210-130 Niteroi, RJ, Brazil. mipwalt@vm.uff.br
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Fluids / microbiology
DNA, Bacterial / analysis,  isolation & purification*
Female
Goats*
Infertility / etiology,  microbiology
Leptospira / genetics,  isolation & purification*
Leptospirosis / complications,  epidemiology,  microbiology,  veterinary*
Male
Polymerase Chain Reaction / methods
Semen / microbiology*
Seroepidemiologic Studies
Sheep*
Vagina / microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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