Document Detail


Detection of La Crosse and snowshoe hare viral nucleic acids by in situ hybridization.
MedLine Citation:
PMID:  2658638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A molecular hybridization technique was developed to detect bunyavirus RNA in cells. Complementary DNAs (cDNAs) to the small (S) RNA segment of La Crosse (LAC) virus and to a portion of the middle (M) RNA segment of snowshoe hare (SSH) virus were used as probes to detect LAC or SSH viral RNA by in situ hybridization. Protocols were developed and standardized using radiolabeled DNA probes, and adapted for use with biotin labeled probes. The in situ hybridization procedure detected an estimated 3,600 copies of viral S RNA/cell at 24 hr postinfection. In growth curve studies, LAC nucleocapsid antigen was detectable slightly before S RNA. LAC S RNA synthesis was first seen about the nucleus. By 12 hr postinfection, hybridization signal was detected throughout the cytoplasm of the cell. The LAC S RNA probe was group-specific and cross-hybridized to 5 other California group viruses. The SSH M RNA probe was type-specific.
Authors:
L J Chandler; B J Beaty; D H Bishop; D C Ward
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  40     ISSN:  0002-9637     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-07-03     Completed Date:  1989-07-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  561-8     Citation Subset:  AIM; IM    
Affiliation:
Colorado State University, Fort Collins.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Viral / analysis
Bunyaviridae / isolation & purification*
Cell Line
DNA Probes
Encephalitis Virus, California / genetics,  immunology,  isolation & purification*
Fluorescent Antibody Technique
Mathematics
Nucleic Acid Hybridization*
Plasmids
Predictive Value of Tests
RNA, Viral / analysis*
Species Specificity
Grant Support
ID/Acronym/Agency:
AI 19688/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Viral; 0/DNA Probes; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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