Document Detail

[Detection of Japanese encephalitis virus in samples of JE patients by RT-PCR]
MedLine Citation:
PMID:  11503059     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To set up and assess the feasibility of RT-PCR method for Japanese encephalitis virus (JEV) detection, and use this method to detect clinical specimens. METHODS: The sensitivity of this RT-PCR was measured by plaque formation test, and the specificity of primers was proved by detecting some other flaviviruses. This RT-PCR was also compared with PRHI. RESULTS: JEV RNA could be detected successfully from viral culturing supernatants and from mouse brain infected by JEV. Its sensitivity of detecting JEV RNA from viral culturing supernatants was 64 PFU. A total of 38 specimens (of which 25 were serum specimens, 13 CSF specimens) from 16 clinically susceptible JE patients were examined by RT-PCR and RPHI Summing up the detection results of RT-PCR and RPHI, 14 patients among the 16 susceptible patients were affirmed as Japanese encephalitis patients. This RT-PCR is specific to JEV. The sensitivity of joint use of RT-PCR and RPHI to detect JEV was 100%, higher than using RPHI alone, and was 7.7% higher than using RT-PCR only. RT-PCR is more suitable for epidemiological survey than virus isolation. CONCLUSIONS: This RT-PCR for JEV detection is highly specific and sensitive. Toxemia period of JE patient is very short, so grasping the time of blood specimen collection will affect virus detection rate.
J Sun; S Tso; B Chen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology     Volume:  14     ISSN:  1003-9279     ISO Abbreviation:  Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2001-08-14     Completed Date:  2003-11-11     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9602873     Medline TA:  Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi     Country:  China    
Other Details:
Languages:  eng     Pagination:  184-7     Citation Subset:  IM    
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
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MeSH Terms
Encephalitis Virus, Japanese / genetics,  isolation & purification*
Encephalitis, Japanese / diagnosis*
Hemagglutination Inhibition Tests
RNA, Viral / blood,  cerebrospinal fluid
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Reg. No./Substance:
0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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