| Detection of JC virus DNA sequences in colorectal cancers in Japan. | |
| | |
MedLine Citation:
|
PMID: 16021515 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
JC virus (JCV), a ubiquitous polyoma virus that commonly infects humans, was first identified as the etiologic agent for the fetal demyelinating disease, progressive multifocal leukoencephalopathy. Recently, a number of reports have documented detection of JCV in samples derived from several types of neural as well as non-neural human tumors. It has been suggested that oncogenicity of JCV depends on a T antigen having a strict structural homology to the T antigen of simian virus 40. To clarify whether JCV might have a potential role with regard to colorectal cancers, we investigated the presence of its genome in a series of cases along with colorectal adenomas and normal colonic mucosa, targeting T antigen, VP and agnoprotein by nested polymerase chain reaction and Southern blotting and T antigen by immunohistochemistry. While VP and agnoprotein were not found in any of the samples examined, T antigen was detected in 6 of 23 colorectal cancers (26.1%) and 1 of 21 adenomas (4.8%), but none of 20 samples of normal colonic mucosa. No clear and diffuse staining with anti-T-antigen antibodies (1:100) could be detected, and there was no correlation with CD20-positive cells, which might have indicated JCV latent infection of B lymphocytes. Presence of T antigen did not influence clinicopathological variables, including survival. In one colonic cancer case positive for T antigen together with lymph node metastasis, DNA extracted from cancer cells in the lymph node revealed no detection of T antigen. Our results are in the intermediate position between the high T antigen rate (81%) in one report and the lack of it (0%) in another focused on colon cancers. It was concluded that T antigen might be integrated in cancer cells in approximately one fourth of Japanese colon cancer cases without clear and diffuse expression of the protein, suggesting a possible role in oncogenesis which might involve a hit-and-run mechanism. |
| | |
Authors:
|
Ryouta Hori; Yoshihiro Murai; Kouichi Tsuneyama; Hekmat Osman Abdel-Aziz; Kazuhiro Nomoto; Hiroyuki Takahashi; Chun-mei Cheng; Tomohiko Kuchina; Brian V Harman; Yasuo Takano |
Related Documents
:
|
11936455 - Immunohistochemical study on expression of alpha-defensin and beta-defensin-2 in human ... 9817965 - Solitary bronchioloalveolar adenoma of the lung. 2427595 - Coexpression of neurofilament and keratin proteins in cutaneous neuroendocrine carcinom... 9990335 - Biochemical analysis of ocular surface mucin abnormalities in dry eye: the canine model. 11936455 - Immunohistochemical study on expression of alpha-defensin and beta-defensin-2 in human ... 21087285 - Agreement among hla antibody detection assays is higher in ever-pregnant donors and imp... |
Publication Detail:
|
Type: Comparative Study; Journal Article Date: 2005-10-19 |
Journal Detail:
|
Title: Virchows Archiv : an international journal of pathology Volume: 447 ISSN: 0945-6317 ISO Abbreviation: Virchows Arch. Publication Date: 2005 Oct |
Date Detail:
|
Created Date: 2005-11-02 Completed Date: 2006-01-18 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9423843 Medline TA: Virchows Arch Country: Germany |
Other Details:
|
Languages: eng Pagination: 723-30 Citation Subset: IM |
Affiliation:
|
First Department of Pathology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama, 930-0152, Japan, ytakano@ms.toyama-mpu.ac.jp. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenocarcinoma
/
pathology,
virology* Adenoma / pathology, virology* Adolescent Aged Aged, 80 and over Antigens, CD20 / metabolism Antigens, Viral, Tumor / analysis Blotting, Southern Colorectal Neoplasms / pathology, virology* DNA, Viral / analysis* Female Humans Immunohistochemistry JC Virus / genetics*, immunology Japan Male Middle Aged Polymerase Chain Reaction Prognosis Tumor Virus Infections / epidemiology* Viral Proteins / analysis Viral Regulatory and Accessory Proteins |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD20; 0/Antigens, Viral, Tumor; 0/DNA, Viral; 0/Viral Proteins; 0/Viral Regulatory and Accessory Proteins; 0/agnoprotein, polyomavirus |
| Comments/Corrections | |
Comment In:
|
Virchows Arch. 2006 Feb;448(2):239
[PMID:
16365728
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Primary clear cell sarcoma of the ileum: an uncommon and misleading site.
Next Document: Learning viewpoint invariant object representations using a temporal coherence principle.