| Detection of inflammatory arthritis by using hyperpolarized 13C-pyruvate with MR imaging and spectroscopy. | |
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MedLine Citation:
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PMID: 21406626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To examine the feasibility of using magnetic resonance (MR) spectroscopy with hyperpolarized carbon 13 ((13)C)-labeled pyruvate to detect inflammation. MATERIALS AND METHODS: The animal care and use committee approved all work with animals. Arthritis was induced in the right hind paw of six rats; the left hind paw served as an internal control. The lactate dehydrogenase-catalyzed conversion of pyruvate to lactate was measured in inflamed and control paws by using (13)C MR spectroscopy. Clinical and histologic data were obtained to confirm the presence and severity of arthritis. Hyperpolarized (13)C-pyruvate was intravenously injected into the rats before simultaneous imaging of both paws with (13)C MR spectroscopy. The Wilcoxon signed rank test was used to test for differences in metabolites between the control and arthritic paws. RESULTS: All animals showed findings of inflammation in the affected paws and no signs of arthritis in the control paws at both visible inspection (clinical index of 3 for arthritic paws and 0 for control paws) and histologic examination (histologic score of 3-5 for arthritic paws and 0 for control paws). Analysis of the spectroscopic profiles of (13)C-pyruvate and converted (13)C-lactate showed an increase in the amount of (13)C-lactate in inflamed paws (median lactate-to-pyruvate ratio, 0.50; mean lactate-to-pyruvate ratio ± standard deviation, 0.52 ± 0.16) versus control paws (median lactate-to-pyruvate ratio, 0.27; mean lactate-to-pyruvate ratio, 0.32 ± 0.11) (P < .03). The ratio of (13)C-lactate to total (13)C was also significantly increased in inflamed paws compared with control paws (P < .03). CONCLUSION: These results suggest that alterations in the conversion of pyruvate to lactate as detected with (13)C-MR spectroscopy may be indicative of the presence of inflammatory arthritis. |
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Authors:
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John D MacKenzie; Yi-Fen Yen; Dirk Mayer; James S Tropp; Ralph E Hurd; Daniel M Spielman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-03-15 |
Journal Detail:
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Title: Radiology Volume: 259 ISSN: 1527-1315 ISO Abbreviation: Radiology Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-19 Completed Date: 2011-07-26 Revised Date: 2012-05-01 |
Medline Journal Info:
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Nlm Unique ID: 0401260 Medline TA: Radiology Country: United States |
Other Details:
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Languages: eng Pagination: 414-20 Citation Subset: AIM; IM |
Copyright Information:
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RSNA, 2011 |
Affiliation:
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Department of Radiology, Stanford University, Stanford, Calif, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arthritis, Experimental / diagnosis*, metabolism Biological Markers / metabolism Carbon Isotopes / diagnostic use, metabolism Contrast Media / diagnostic use, metabolism Disease Models, Animal Heterocyclic Compounds / diagnostic use, metabolism Inflammation / diagnosis, metabolism Lactic Acid / metabolism Magnetic Resonance Spectroscopy / instrumentation, methods* Organometallic Compounds / diagnostic use, metabolism Pyruvates / diagnostic use*, metabolism* Rats Rats, Sprague-Dawley Statistics, Nonparametric |
| Grant Support | |
ID/Acronym/Agency:
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2674-2//PHS HHS; AA018681/AA/NIAAA NIH HHS; EB009070/EB/NIBIB NIH HHS; R01 EB009070-03/EB/NIBIB NIH HHS; R01 EB009070-04/EB/NIBIB NIH HHS; RR09784/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Carbon Isotopes; 0/Contrast Media; 0/Heterocyclic Compounds; 0/Organometallic Compounds; 0/Pyruvates; 112188-16-6/gadoteridol; 50-21-5/Lactic Acid |
| Comments/Corrections | |
Comment In:
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Radiology. 2011 May;259(2):309-10
[PMID:
21502386
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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