| Detection of ICAM-1 in experimentally induced colitis of ICAM-1-deficient and wild-type mice: an immunohistochemical study. | |
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MedLine Citation:
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PMID: 11254085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adhesion molecules (e.g. ICAM-1, CD 54) are known to be upregulated on activated vascular endothelial cells during inflammatory reactions. To study the role of ICAM-1 in intestinal inflammation in vivo, we induced acute experimental colitis in wild-type (C57BL/6) mice and ICAM-1-deficient mice, by feeding the animals with 3% dextran sodium sulphate (DSS) in drinking water for 7 days. In the control strain the immunohistochemical staining showed a very pronounced endothelial upregulation of ICAM-1 after the DSS treatment observed in areas of inflammatory infiltrate, especially in venules or arterioles of the propria and submucosa, and partly in the mesocolon. DSS-fed ICAM-1-deficient mice showed no endothelial enhancement and only faint staining of venules or capillaries approaching that encountered in the control ICAM-1-deficient animals. Our data indicate that ICAM-1 may play a crucial role in the development of acute intestinal inflammation, consistent with our finding that ICAM-1 deficiency can obviate severe forms of experimentally induced colitis in mice. |
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Authors:
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F Bendjelloul; P Rossmann; P Malý; V Mandys; M Jirkovská; L Prokesová; L Tucková; H Tlaskalová-Hogenová |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Histochemical journal Volume: 32 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-03-19 Completed Date: 2001-07-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: Netherlands |
Other Details:
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Languages: eng Pagination: 703-9 Citation Subset: IM |
Affiliation:
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Division of Immunology and Gnotobiology, Institute of Microbiology, ASCR, Prague, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Colitis / chemically induced, immunology, metabolism* Colon / chemistry Dextran Sulfate Endothelium, Vascular / metabolism Female Immunohistochemistry Inflammation / metabolism, pathology Intercellular Adhesion Molecule-1 / analysis, drug effects, physiology* Mice Mice, Inbred C57BL Mice, Mutant Strains Up-Regulation |
| Chemical | |
Reg. No./Substance:
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126547-89-5/Intercellular Adhesion Molecule-1; 9042-14-2/Dextran Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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