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Detection of the I1781L mutation in fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.) based on the loop-mediated isothermal amplification (LAMP) method.
MedLine Citation:
PMID:  24644047     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: The increasing use of fenoxaprop-P-ethyl has resulted in evolved resistance in American sloughgrass (Beckmannia syzigachne Steud.). Target site-based resistance to acetyl-CoA carboxylase (ACCase) inhibitors in B. syzigachne occurs due to an isoleucine-to-leucine substitution at residue 1781 (I1781L) of the ACCase enzyme. A rapid detection method is needed to identify the resistance-conferring substitution.
RESULTS: Four populations of B. syzigachne that were resistant to fenoxaprop-P-ethyl and contained the I1781L substitution were identified. Conventional PCR and derived cleaved amplified polymorphic sequence (dCAPS) methods were used to detect the mutation. Additionally, a rapid nucleic acid detection method, loop-mediated isothermal amplification (LAMP), was successfully developed and used to detect the genetic mutation underlying the I1781L substitution in the B. syzigachne ACCase enzyme.
CONCLUSION: This report is the first to describe the application of a LAMP assay for mutation detection in herbicide-resistant weeds. The assay does not require specialized equipment: only a standard laboratory bath is needed. This technique could be employed for detecting the I1781L substitution in B. syzigachne plants and seeds.
Authors:
Lang Pan; Jun Li; Wen-Na Zhang; Liyao Dong
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-18
Journal Detail:
Title:  Pest management science     Volume:  -     ISSN:  1526-4998     ISO Abbreviation:  Pest Manag. Sci.     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100898744     Medline TA:  Pest Manag Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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This article is protected by copyright. All rights reserved.
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