Document Detail


Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.
MedLine Citation:
PMID:  17276371     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.
Authors:
Karen Yao; Nahid Akyani; Donatella Donati; Naomi Sengamalay; Julie Fotheringham; Elodie Ghedin; Michael Bishop; John Barrett; Fatah Kashanchi; Steven Jacobson
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  37 Suppl 1     ISSN:  1386-6532     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-02-05     Completed Date:  2007-08-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  S57-62     Citation Subset:  IM    
Affiliation:
Viral Immunology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow Transplantation*
Brain / virology*
Brain Diseases / virology*
DNA, Viral / analysis,  genetics
Herpesvirus 6, Human / classification,  genetics,  isolation & purification*
Humans
Polymerase Chain Reaction
RNA, Viral / biosynthesis,  genetics
Roseolovirus Infections / virology*
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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