| Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis. | |
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MedLine Citation:
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PMID: 17276371 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT. |
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Authors:
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Karen Yao; Nahid Akyani; Donatella Donati; Naomi Sengamalay; Julie Fotheringham; Elodie Ghedin; Michael Bishop; John Barrett; Fatah Kashanchi; Steven Jacobson |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Volume: 37 Suppl 1 ISSN: 1386-6532 ISO Abbreviation: J. Clin. Virol. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2007-02-05 Completed Date: 2007-08-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815671 Medline TA: J Clin Virol Country: Netherlands |
Other Details:
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Languages: eng Pagination: S57-62 Citation Subset: IM |
Affiliation:
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Viral Immunology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bone Marrow Transplantation* Brain / virology* Brain Diseases / virology* DNA, Viral / analysis, genetics Herpesvirus 6, Human / classification, genetics, isolation & purification* Humans Polymerase Chain Reaction RNA, Viral / biosynthesis, genetics Roseolovirus Infections / virology* Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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