Document Detail


Detection of HCV and GBV-C/HGV infection by multiplex PCR in plasma samples of transfused subjects.
MedLine Citation:
PMID:  9506815     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A multiplex polymerase chain reaction (PCR) was applied to clinical samples for simultaneous detection of hepatitis C virus (HCV) and GBV-C/HGV genome. With both RNA viruses, the amplification was performed with primers of the 5' UTR region starting from the single viral RNA reverse transcripted (cDNA) with random hexanucleotide primer mix. GBV-C/HGV RNA was detected in plasma sample of seven out of 50 transfused patients (14%). The multiplex PCR demonstrated a sensitivity up to 7.8 x 10(2) copies/ml respectively for GBV-C/HGV and HCV RNA in plasma samples of 5/50 patients with GBV-C/HGV/HCV co-infection and in patients with HCV (27/50) or GBV-C/HGV infection alone (2/50).
Authors:
C Caudai; M G Padula; V Bettini; P E Valensin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virological methods     Volume:  70     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-04-28     Completed Date:  1998-04-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  79-83     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Microbiology Section, University of Siena, Italy.
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MeSH Terms
Descriptor/Qualifier:
Blood Transfusion*
Flaviviridae* / genetics,  isolation & purification
Genome, Viral
Hepacivirus / genetics,  isolation & purification
Hepatitis C / complications,  diagnosis*
Hepatitis, Viral, Human / complications,  diagnosis*
Humans
Polymerase Chain Reaction*
RNA, Viral / blood
Sensitivity and Specificity
Transcription, Genetic
Chemical
Reg. No./Substance:
0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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