Document Detail


Detection of free peritoneal cancer cells in gastric cancer using cancer-specific Newcastle disease virus.
MedLine Citation:
PMID:  19902312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Cytologic detection of peritoneal gastric cancer cells by Papanicolaou staining offers important prognostic information but has low sensitivity. We evaluated a novel detection technique using Newcastle disease virus expressing the enhanced green fluorescent protein (NDV-GFP) gene.
METHODS: NDV-GFP was tested on MKN-1 human gastric adenocarcinoma cells plated upon rat hepatocytes to determine tumor-specific infection and GFP expression. Malignant ascites infected with increasing doses of virus was evaluated for NDV-GFP dose determination. Peritoneal lavage samples from 30 patients with gastric adenocarcinoma undergoing staging laparoscopy were evaluated with NDV-GFP.
RESULTS: NDV-GFP can specifically detect one MKN-1 cell among one million benign rat hepatocytes. NDV-GFP at 5 x 10(6) plaque-forming units (PFU) produced optimal GFP expression in malignant ascites. Noncancerous cells were non-GFP expressing. GFP-expressing cells counterstained positive for carcinoembryonic antigen expression, confirming their cancerous origin. Furthermore, in patients with advanced gastric cancer, GFP expression was markedly enhanced over cytology. Of six patients with M1 disease discovered during laparoscopy, only 50% were cytology positive. All six, however, were NDV-GFP positive. Cytology was positive in 9% of patients with T3 disease, 8% with N1 disease, and 50% with N2 disease. In contrast, NDV-GFP was positive in 95% of T3 patients and 100% of patients with N1 or N2 disease.
CONCLUSIONS: NDV-GFP can specifically infect and detect peritoneal gastric cancer cells and offers a more sensitive method compared with conventional cytology. This novel modality may offer enhanced detection of intraperitoneal cancer spread and provide important prognostic information.
Authors:
Joyce Wong; Allison Schulman; Kaitlyn Kelly; Dmitriy Zamarin; Peter Palese; Yuman Fong
Publication Detail:
Type:  Journal Article     Date:  2009-11-10
Journal Detail:
Title:  Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract     Volume:  14     ISSN:  1873-4626     ISO Abbreviation:  J. Gastrointest. Surg.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-05-10     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  9706084     Medline TA:  J Gastrointest Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / pathology*,  secondary
Animals
Ascitic Fluid / pathology*
Carcinoembryonic Antigen / analysis
Cell Line, Tumor
Cells, Cultured
Fluorescent Antibody Technique
Green Fluorescent Proteins / diagnostic use*,  genetics
Hepatocytes
Humans
Male
Microscopy, Fluorescence
Newcastle disease virus / genetics*
Peritoneal Lavage
Peritoneal Neoplasms / pathology,  secondary*
Rats
Rats, Sprague-Dawley
Stomach Neoplasms / pathology*
Grant Support
ID/Acronym/Agency:
T32 CA009501/CA/NCI NIH HHS; T32 CA009501-21/CA/NCI NIH HHS; T32 CA009501-22/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Carcinoembryonic Antigen; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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