Document Detail

Detection of fetal cells in the maternal kidney during gestation in the mouse.
MedLine Citation:
PMID:  19478466     Owner:  NLM     Status:  MEDLINE    
It has been reported that fetal cells migrate into maternal blood and organs. Since these fetal chimeric cells could be involved in maternal allogeneic tolerance to the fetus, the fetal chimeric cells might be implicated in maternal-fetal immunology and development of maternal autoimmune diseases. However, the mechanism and role of fetal microchimerism remains unclear. We aimed to describe the mechanism by which fetal cells become associated with maternal organs during pregnancy, using a mouse fetal microchimerism model. Non-obese diabetic/severe combined immunodeficiency (NOD/SCID) female mice, which are useful for tracking the behavior of fetal cells in the maternal body, were mated with transgenic males expressing enhanced green fluorescent protein (GFP), and the presence of GFP-positive cells were examined in peripheral blood and organs of pregnant mothers. By flow cytometry, we showed that 0.95 +/- 0.48% of mononuclear cells detected in the maternal peripheral blood were GFP-positive, and thus of fetal origin, during the first gestational week. This value decreased to 0.10 +/- 0.13% during the third gestational week (p < 0.05). GFP-positive cells were detected in the extraglomerular mesangial region and among the epithelial cells of the proximal renal tubule of the maternal kidney. These GFP-positive cells also expressed angiotensin II receptor subtype 2 (AT2), which is known to participate in regulating organogenesis and vasoreactivity. Fetal cells expressing AT2 may therefore be involved in the regulation of vascular tone in the maternal kidney. These observations suggest that fetal cells could influence maternal renal function through activation of the AT2 signaling.
Keiichi Matsubara; Naoyuki Uchida; Yuko Matsubara; Shinji Hyodo; Masaharu Ito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Tohoku journal of experimental medicine     Volume:  218     ISSN:  1349-3329     ISO Abbreviation:  Tohoku J. Exp. Med.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-29     Completed Date:  2009-08-13     Revised Date:  2013-01-03    
Medline Journal Info:
Nlm Unique ID:  0417355     Medline TA:  Tohoku J Exp Med     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  107-13     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Ehime University School of Medicine, Ehime, Japan.
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MeSH Terms
Cell Separation
Fetus / cytology*
Flow Cytometry
Green Fluorescent Proteins / metabolism
Kidney / cytology*
Mice, Inbred NOD
Mice, SCID
Organ Specificity
Pregnancy / blood,  physiology*
Receptor, Angiotensin, Type 1 / metabolism
Receptor, Angiotensin, Type 2 / metabolism
Reg. No./Substance:
0/Receptor, Angiotensin, Type 1; 0/Receptor, Angiotensin, Type 2; 147336-22-9/Green Fluorescent Proteins

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