| Detection of Fasciola-specific excretory/ secretory (E/S) protein fraction band (49.5 kDa) and its utilization in diagnosis of early fascioliasis using different diagnostic techniques. | |
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MedLine Citation:
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PMID: 12561930 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of the present work is to evaluate Fasciola E/S antigens for diagnosis of early fascioliasis utilizing different diagnostic techniques. Using enzyme-linked immunoelectro-transfer blot (EITB), Fasciola-specific E/ S protein fraction band (49.5 kDa) was determined and electroeluted. The mono-specific antibodies against this specific fraction band were prepared by immunizing pathogen-free rabbit. Assessment of the prepared mono-specific antibodies in diagnosis of human fascioliasis was performed through the detection of E/S copro-antigens by enzyme-linked immunosorbent assay (ELISA) in stool eluates obtained from patients with confirmed fascioliasis, other parasites as well as from other healthy individuals. Serum samples were collected and tested to detect serum antibodies against Fasciola E/S antigen using EITB and counter immunoelectrophoresis (CIEP). Analysis of Fasciola adult worm E/S products by SDS/PAGE revealed a number of bands, the molecular weight (MW) of which ranged from 14-200 kDa; with three major bands (27.5, 32.5 and 55 kDa). Fasciola EIS 49.5 kDa protein fraction proved to be specific to F. gigantica. Cross reaction with S. mansoni was observed at higher MW (110-120 kDa). The sensitivity, specificity and diagnostic accuracy of EITB were 45.2%, 100% and 70.7%, respectively, while those of CIEP were 38.7%, 100% and 67.2%, respectively. ELISA technique using mono-specific anti-49.5 kDa to detect copro-antigens proved to be practical and reliable. It showed higher sensitivity (91.4%) and higher diagnostic accuracy (91.8%), while the specificity was 92.3%. In addition, ELISA had higher negative predictive value (88.9%) and fair positive predictive value (94.1%). |
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Authors:
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M S Arafa; S M Abaza; K A El-Shewy; E W Mohareb; A A El-Moamly |
Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Journal of the Egyptian Society of Parasitology Volume: 29 ISSN: 1110-0583 ISO Abbreviation: J Egypt Soc Parasitol Publication Date: 1999 |
Date Detail:
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Created Date: 2003-02-03 Completed Date: 2003-02-27 Revised Date: 2013-05-02 |
Medline Journal Info:
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Nlm Unique ID: 8102141 Medline TA: J Egypt Soc Parasitol Country: Egypt |
Other Details:
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Languages: eng Pagination: 911-26 Citation Subset: IM |
Affiliation:
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Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Helminth / blood Antigens, Helminth / chemistry, diagnostic use*, immunology Counterimmunoelectrophoresis Enzyme-Linked Immunosorbent Assay Fasciola / immunology* Fasciola hepatica / immunology Fascioliasis / diagnosis*, parasitology Feces / parasitology Helminth Proteins / chemistry, diagnostic use*, immunology Humans Immunoblotting / methods Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Helminth; 0/Antigens, Helminth; 0/Helminth Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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