| Detection of Escherichia coli Shiga toxin (stx) and enterotoxin (estA and elt) genes in fecal samples from non-diarrheic and diarrheic greyhounds. | |
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MedLine Citation:
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PMID: 12829384 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Virulence factors responsible for acute diarrhea in greyhounds have not been well established. The objective of this study was to determine if a correlation exists between disease and the presence of the Escherichia coli toxin genes in non-diarrheic and diarrheic greyhound feces. DNA extracted from broth cultures was evaluated for the presence of Shiga toxin and enterotoxin genes and broth samples were evaluated for Shiga toxin and heat-labile enterotoxin. Shiga toxin (stx1 and stx2) and enterotoxin (et and estA) genes were identified in both non-diarrheic and diarrheic samples after in vitro cultured of swabs at 37 degrees C for 16-24h. The stx1 gene was present in 3% of non-diarrheic and 15% diarrheic samples and the stx2 gene was identified in 36 and 23%, non-diarrheic and diarrheic samples, respectively. Shiga toxin was present in 48% diarrheic and 25% of the non-diarrheic in vitro cultured samples. The elt gene was detected in vitro cultured swabs in 12% of the non-diarrheic and 7% of the diarrheic samples. Labile toxin was present in the feces of small numbers of both groups of dogs. A significant correlation existed between the presence of both stx1 genes and Shiga toxin in feces, and lack of disease in non-diarrheic (P=0.01) and presence of disease in diarrheic (P=0.024) greyhounds. Correlation between production of Shiga toxin and detection of stx1 or stx2 was significant in both the diarrheic and non-diarrheic feces (P=0.03); however, only the presence of stx1 correlated with diarrhea in both groups of samples (P<0.008). The incidence of toxigenic E. coli in both non-diarrheic and diarrheic greyhounds indicates a zoonotic potential from dogs to humans and requires further study. |
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Authors:
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Jacque J Staats; M M Chengappa; Mary C DeBey; Barry Fickbohm; Richard D Oberst |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Veterinary microbiology Volume: 94 ISSN: 0378-1135 ISO Abbreviation: Vet. Microbiol. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-06-27 Completed Date: 2003-10-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705469 Medline TA: Vet Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 303-12 Citation Subset: IM |
Affiliation:
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Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Animals Bacterial Toxins / chemistry, genetics* DNA, Bacterial / isolation & purification* Diarrhea / microbiology, veterinary* Dog Diseases / microbiology* Dogs Enterotoxins / chemistry, genetics Escherichia coli / pathogenicity* Feces / microbiology Genes, Bacterial Nucleic Acid Hybridization Polymerase Chain Reaction / veterinary Shiga Toxin 1 / chemistry, genetics Shiga Toxin 2 / chemistry, genetics Virulence / genetics |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/DNA, Bacterial; 0/Enterotoxins; 0/Shiga Toxin 1; 0/Shiga Toxin 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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