| Detection of the EGFP sequence in breast muscle of 3-year-old chicken after transfection using sonoporation. | |
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MedLine Citation:
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PMID: 21615836 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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In our continuing effort to generate transgenic chickens, sonoporation was chosen to insert an exogenous gene into the chicken genome. An EGFP expression vector (pCAG-EGFPac) and microbubbles were injected into the central disc of stage-X blastoderm or the germinal crescent of stage-4 embryos, followed by ultrasonic vibration. Nineteen chicks out of 108 treated embryos hatched, six females and six males out of these 19 chicks grew to sexual maturity and two females and three males lived for 3 years. Genomic DNA from 17 out of 35 gonads from embryos and chicks that died before sexual maturity was EGFP-positive by PCR. No EGFP sequence was detected in the genomic DNA of 322 embryos from six sexually mature females and the semen from four sexually mature males by PCR. When genomic DNA was obtained from various tissues of five 3-year-old chickens, the EGFP sequence was amplified from the genomic DNA of the breast muscle of a female (No. 85). The above sequence was subjected to DNA sequencing and verified to be the EGFP sequence. These results showed that sonoporation is an effective tool for the transduction of exogenous genes into chicken embryos for the generation of transgenic chickens. |
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Authors:
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Yuko Matsubara; Takahiroi Tagami; Daisuke Matsunaga; Akiko Sano; Kimiaki Maruyama |
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Publication Detail:
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Type: Journal Article Date: 2011-03-06 |
Journal Detail:
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Title: Animal science journal = Nihon chikusan Gakkaihō Volume: 82 ISSN: 1740-0929 ISO Abbreviation: Anim. Sci. J. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100956805 Medline TA: Anim Sci J Country: Australia |
Other Details:
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Languages: eng Pagination: 428-33 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Animal Science Journal © 2011 Japanese Society of Animal Science. |
Affiliation:
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Transgenic Animal Research Center, National Institute of Agrobiological Sciences National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki Biological Science, Meiji University, Kawasaki, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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