Document Detail


Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA.
MedLine Citation:
PMID:  1854600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.
Authors:
K S Cudjoe; L I Thorsen; T Sørensen; J Reseland; O Olsvik; P E Granum
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  12     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-08-23     Completed Date:  1991-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  313-21     Citation Subset:  IM    
Affiliation:
MATFORSK, Norwegian Food Research Institute, As.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Clostridium perfringens*
Enterotoxins / analysis*
Enzyme-Linked Immunosorbent Assay / methods*
Feces / chemistry*
Food Analysis / methods*
Humans
Immunoelectrophoresis, Two-Dimensional
Microspheres
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Enterotoxins; 0/enterotoxin, Clostridium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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