Document Detail

Detection and Characterization of Shigella species Isolated from Food and Human stool samples in Nabeul, Tunisia by Molecular Methods and Culture Techniques.
MedLine Citation:
PMID:  22548495     Owner:  NLM     Status:  Publisher    
Aims:  the present study was designed to isolate Shigella spp. strains from food and stool samples by a combination of PCR and culture methods and characterize their serotypes, antibiotic resistance profiles, virulence genes and pulsed-field gel electrophoresis patterns to investigate possible clonal relationships among strains circulating. Methods and Results:  Six Shigella spp. strains were isolated from 280 food samples against 16 Shigella isolates from 236 stool samples of symptomatic patients and asymptomatic food handlers during the period from January 2007 to December 2009 in Public Health Regional Laboratory of Nabeul. The detection of ipaH, ipaBCD, ial, ShET-1 and ShET-2 was performed by a PCR technique with specific primers. Conclusions:  The use of PCR technique improved the rate of detecting Shigella in stool samples from 6.7% to 14% and in food samples from 2.1% to 8.6%. Percentage of Shigella isolates and ipaH specific PCR demonstrated a marked pattern of seasonality, increasing in summer and fall seasons for human and food isolates. Among the environmental strains, 50% of isolates were invasive. However for the 16 clinical strains isolated, nine were found to be positive for both ial and ipaBCD gene and 11 were found to produce ShET-1 and/or ShET-2. XbaI PFGE analysis revealed the presence of a predominant clone among Sh. sonnei strains recovered from different sources circulating in Nabeul, Tunisia throughout the years 2007-2009. Significance and Impact of the study:  This study demonstrated the existence of Shigella in food samples and dispersion of different virulence genes among these isolates, which appear to constitute an environmental source of epidemic spread. The clonal relationships among strains isolated from food elements and human stools indicate the incrimination of different kinds of foods as vehicle of transmission of Shigella, which are usually escaped from detection by traditional culture methods. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
W Mokhtari; S Nsaibia; D Majouri; A Ben Hassen; A Gharbi; M Aouni
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-30
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-5-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Laboratoire Régional de Santé Publique de Nabeul, Tunisie. Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives (LR99-ES27), Faculté de Pharmacie de Monastir, Tunisie. Service des Laboratoires, Centre Nationale de Greffe de la Moelle Osseuse, Tunis.
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