Document Detail


Detection of Campylobacter jejuni and Campylobacter coli in foods by enrichment culture and polymerase chain reaction enzyme-linked immunosorbent assay.
MedLine Citation:
PMID:  12030285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A polymerase chain reaction (PCR) assay based on a solution hybridization format with colorimetric end-point detection (PCR ELISA) was investigated for the specific detection of Campylobacter jejuni and Campylobacter coli in food samples following enrichment culture. One hundred fifteen samples of raw meat and offal (poultry, porcine, ovine, and bovine), raw shellfish, and artificially contaminated milk were enriched in blood-free Campylobacter Enrichment Broth for 48 h. Enrichment cultures were subcultured to Campylobacter blood-free selective agar plates, and presumptive isolates were identified by phenotypic methods. DNA was extracted from 1-ml aliquots of the enrichment cultures using a rapid extraction method, and the DNA was used as the template in a PCR ELISA. A comparison of the PCR ELISA with the enrichment culture and subculture to selective agar method showed that the results of 112 of the 115 samples tested were in agreement by both methods. Seventy-one of the various food samples were positive in the PCR ELISA, and 70 samples were positive by culture. The PCR ELISA had a sensitivity of 99% and a specificity of 96%, with a positive predictive value of 97% and a negative predictive value of 98%. The PCR ELISA is a rapid, sensitive, and specific method for the detection of C. jejuni and C. coli in foods following enrichment culture and significantly reduces the time required for their detection.
Authors:
F J Bolton; A D Sails; A J Fox; D R A Wareing; D L A Greenway
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  65     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-27     Completed Date:  2002-07-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  760-7     Citation Subset:  IM    
Affiliation:
Preston Public Health Laboratory, Royal Preston Hospital, Lancashire, UK. fbolton@PHLS.org.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Campylobacter coli / genetics,  isolation & purification*
Campylobacter jejuni / genetics,  isolation & purification*
Cattle
Chickens
Culture Media
DNA, Bacterial / analysis*
Enzyme-Linked Immunosorbent Assay / methods
Food Microbiology
Meat / microbiology*
Milk / microbiology*
Polymerase Chain Reaction / methods
Predictive Value of Tests
Sensitivity and Specificity
Sheep
Shellfish / microbiology*
Swine
Chemical
Reg. No./Substance:
0/Culture Media; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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