| Detection of BrdU-label retaining cells in the lacrimal gland: implications for tissue repair. | |
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MedLine Citation:
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PMID: 22101331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of the present study was to determine if the lacrimal gland contains 5-bromo-2'-deoxyuridine (BrdU)-label retaining cells and if they are involved in tissue repair. Animals were pulsed daily with BrdU injections for 7 consecutive days. After a chase period of 2, 4, or 12 weeks, the animals were sacrificed and the lacrimal glands were removed and processed for BrdU immunostaining. In another series of experiments, the lacrimal glands of 12-week chased animals were either left untreated or were injected with interleukin 1 (IL-1) to induce injury. Two and half days post-injection, the lacrimal glands were removed and processed for BrdU immunostaining. After 2 and 4 weeks of chase period, a substantial number of lacrimal gland cells were BrdU(+) (11.98 ± 1.84 and 7.95 ± 1.83 BrdU(+) cells/mm(2), respectively). After 12 weeks of chase, there was a 97% decline in the number of BrdU(+) cells (0.38 ± 0.06 BrdU(+) cells/mm(2)), suggesting that these BrdU-label retaining cells may represent slow-cycling adult stem/progenitor cells. In support of this hypothesis, the number of BrdU labeled cells increased over 7-fold during repair of the lacrimal gland (control: 0.41 ± 0.09 BrdU(+) cells/mm(2); injured: 2.91 ± 0.62 BrdU(+) cells/mm(2)). Furthermore, during repair, among BrdU(+) cells 58.2 ± 3.6 % were acinar cells, 26.4 ± 4.1% were myoepithelial cells, 0.4 ± 0.4% were ductal cells and 15.0 ± 3.0% were stromal cells. We conclude that the murine lacrimal gland contains BrdU-label retaining cells that are mobilized following injury to generate acinar, myoepithelial and ductal cells. |
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Authors:
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Samantha You; Ayesha Tariq; Claire L Kublin; Driss Zoukhri |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-11-19 |
Journal Detail:
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Title: Cell and tissue research Volume: 346 ISSN: 1432-0878 ISO Abbreviation: Cell Tissue Res. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-23 Completed Date: 2012-07-16 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 317-26 Citation Subset: IM |
Affiliation:
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Department of General Dentistry, Tufts University School of Dental Medicine, Boston, MA 0211, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bromodeoxyuridine / administration & dosage, analysis*, pharmacokinetics Female Lacrimal Apparatus / chemistry*, cytology, metabolism, physiology* Mice Mice, Inbred BALB C Tissue Distribution Wound Healing / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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P30 NS047243/NS/NINDS NIH HHS; R01 EY012383-09A2/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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