Document Detail

Detection of BrdU-label retaining cells in the lacrimal gland: implications for tissue repair.
MedLine Citation:
PMID:  22101331     Owner:  NLM     Status:  MEDLINE    
The purpose of the present study was to determine if the lacrimal gland contains 5-bromo-2'-deoxyuridine (BrdU)-label retaining cells and if they are involved in tissue repair. Animals were pulsed daily with BrdU injections for 7 consecutive days. After a chase period of 2, 4, or 12 weeks, the animals were sacrificed and the lacrimal glands were removed and processed for BrdU immunostaining. In another series of experiments, the lacrimal glands of 12-week chased animals were either left untreated or were injected with interleukin 1 (IL-1) to induce injury. Two and half days post-injection, the lacrimal glands were removed and processed for BrdU immunostaining. After 2 and 4 weeks of chase period, a substantial number of lacrimal gland cells were BrdU(+) (11.98 ± 1.84 and 7.95 ± 1.83 BrdU(+) cells/mm(2), respectively). After 12 weeks of chase, there was a 97% decline in the number of BrdU(+) cells (0.38 ± 0.06 BrdU(+) cells/mm(2)), suggesting that these BrdU-label retaining cells may represent slow-cycling adult stem/progenitor cells. In support of this hypothesis, the number of BrdU labeled cells increased over 7-fold during repair of the lacrimal gland (control: 0.41 ± 0.09 BrdU(+) cells/mm(2); injured: 2.91 ± 0.62 BrdU(+) cells/mm(2)). Furthermore, during repair, among BrdU(+) cells 58.2 ± 3.6 % were acinar cells, 26.4 ± 4.1% were myoepithelial cells, 0.4 ± 0.4% were ductal cells and 15.0 ± 3.0% were stromal cells. We conclude that the murine lacrimal gland contains BrdU-label retaining cells that are mobilized following injury to generate acinar, myoepithelial and ductal cells.
Samantha You; Ayesha Tariq; Claire L Kublin; Driss Zoukhri
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-11-19
Journal Detail:
Title:  Cell and tissue research     Volume:  346     ISSN:  1432-0878     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-23     Completed Date:  2012-07-16     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  317-26     Citation Subset:  IM    
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MeSH Terms
Bromodeoxyuridine / administration & dosage,  analysis*,  pharmacokinetics
Lacrimal Apparatus / chemistry*,  cytology,  metabolism,  physiology*
Mice, Inbred BALB C
Tissue Distribution
Wound Healing / physiology*
Grant Support
P30 NS047243/NS/NINDS NIH HHS; R01 EY012383/EY/NEI NIH HHS; R01 EY012383-09A2/EY/NEI NIH HHS
Reg. No./Substance:

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