Document Detail

Detection of Ara h 1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor.
MedLine Citation:
PMID:  25173734     Owner:  NLM     Status:  Publisher    
A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter=32nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody-antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3h 50min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000ng/ml, with a limit of detection of 3.8ng/ml, and provided precise (RSD <8.7%) and accurate (recovery >96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.
Rita C Alves; Filipa B Pimentel; Henri P A Nouws; Raquel C B Marques; María Begoña González-García; M Beatriz P P Oliveira; Cristina Delerue-Matos
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-19
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  64C     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  19-24     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier B.V.
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