| Detection of an 85,000-dalton phosphoprotein in ts110 murine sarcoma virus-infected cells with antiserum against a v-mos peptide. | |
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MedLine Citation:
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PMID: 6300455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have used an antiserum directed against a synthetic v-mos peptide (anti-C3 serum) to screen ts110 murine sarcoma virus (MuSV)-infected cells for the presence of v-mos-encoded proteins. Anti-C3 serum specifically recognized an 85,000-dalton protein doublet (P85) from [35S]methionine-labeled ts110 MuSV-infected producer cells grown at 32 degrees C, the permissive temperature for transformation. The P85 doublet was also recognized by an antiserum directed against the viral gag protein p15. P85 was present but at 2- to 10-fold-lower levels in ts110 MuSV-infected producer cells grown at 39 degrees C, the restrictive temperature for transformation. The P85gag-mos fusion product was the only v-mos protein reproducibly detected in this ts110 MuSV-transformed cell line. Immunoprecipitation of 32P-labeled cells with anti-C3 serum revealed that the upper band of the P85 doublet is phosphorylated, containing mostly phosphoserine and some phosphothreonine. Cells acutely infected with ts110 MuSV contained slightly higher levels of P85 than did the ts110 MuSV-infected producer cell line. Anti-C3 serum specifically recognized a 33,000-dalton protein (p33) in the acutely infected cells labeled with [35S]methionine. p33 was present in trace amounts and may represent a previously unidentified ts110 MuSV-encoded v-mos protein. |
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Authors:
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J Papkoff; T Hunter |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 45 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-05-27 Completed Date: 1983-05-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1177-82 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Viral / immunology Antibody Specificity Defective Viruses / analysis* Electrophoresis, Polyacrylamide Gel Gene Products, gag Mice Moloney murine leukemia virus / analysis* Phosphoserine / analysis Phosphothreonine / analysis Rats Sarcoma Viruses, Murine / analysis* Viral Core Proteins Viral Proteins / analysis*, immunology |
| Grant Support | |
ID/Acronym/Agency:
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CA14195/CA/NCI NIH HHS; CA17096/CA/NCI NIH HHS; CA28458/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Gene Products, gag; 0/Viral Core Proteins; 0/Viral Proteins; 1114-81-4/Phosphothreonine; 17885-08-4/Phosphoserine |
| Comments/Corrections | |
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