Document Detail


Detection of a 140 KDA nucleolar protein with an anti-PCNA autoantibody.
MedLine Citation:
PMID:  2858268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess the numbers and types of PCNA (proliferating cell nuclear antigen) species, immunoprecipitation studies on HeLa cell, nuclear and nucleolar extracts were performed. A 140 KDa protein from HeLa nucleoli was immunoprecipitated by an autoantibody (E.B.) previously used to detect the proliferating cell nuclear antigens (PCNA). The 140 KDa protein was also detected in the nuclear extract of colon carcinoma cells (omega) labeled in vitro with 125I-Bolton Hunter reagent. When the growth of the colon carcinoma cells was inhibited by 1% N,N-dimethylformamide for two weeks, the 140 KDa protein was not detected which suggests this protein is associated with cell growth.
Authors:
P K Chan; M Aldrich; E M Tan; H Busch
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell biology international reports     Volume:  9     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-04-26     Completed Date:  1985-04-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  61-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Autoantibodies / immunology*
Cell Division
Cells, Cultured
Colonic Neoplasms / immunology
Hela Cells / immunology
Humans
Molecular Weight
Nuclear Proteins*
Nucleoproteins / immunology*,  isolation & purification
Proliferating Cell Nuclear Antigen
Ribonucleoproteins / immunology*
Grant Support
ID/Acronym/Agency:
CA-10893/CA/NCI NIH HHS; CA-27534/CA/NCI NIH HHS; P1//PHS HHS
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Nuclear Proteins; 0/Nucleoproteins; 0/Proliferating Cell Nuclear Antigen; 0/Ribonucleoproteins; 117896-08-9/nucleophosmin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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