Document Detail


Detecting predation and scavenging by DNA gut-content analysis: a case study using a soil insect predator-prey system.
MedLine Citation:
PMID:  15517409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
White grubs (larvae of Coleoptera: Scarabaeidae) are abundant in below-ground systems and can cause considerable damage to a wide variety of crops by feeding on roots. White grub populations may be controlled by natural enemies, but the predator guild of the European species is barely known. Trophic interactions within soil food webs are difficult to study with conventional methods. Therefore, a polymerase chain reaction (PCR)-based approach was developed to investigate, for the first time, a soil insect predator-prey system. Can, however, highly sensitive detection methods identify carrion prey in predators, as has been shown for fresh prey? Fresh Melolontha melolontha (L.) larvae and 1- to 9-day-old carcasses were presented to Poecilus versicolor Sturm larvae. Mitochondrial cytochrome oxidase subunit I fragments of the prey, 175, 327 and 387 bp long, were detectable in 50% of the predators 32 h after feeding. Detectability decreased to 18% when a 585 bp sequence was amplified. Meal size and digestion capacity of individual predators had no influence on prey detection. Although prey consumption was negatively correlated with cadaver age, carrion prey could be detected by PCR as efficiently as fresh prey irrespective of carrion age. This is the first proof that PCR-based techniques are highly efficient and sensitive, both in fresh and carrion prey detection. Thus, if active predation has to be distinguished from scavenging, then additional approaches are needed to interpret the picture of prey choice derived by highly sensitive detection methods.
Authors:
Anita Juen; Michael Traugott
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-10-29
Journal Detail:
Title:  Oecologia     Volume:  142     ISSN:  0029-8549     ISO Abbreviation:  Oecologia     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-18     Completed Date:  2005-03-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0150372     Medline TA:  Oecologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  344-52     Citation Subset:  IM    
Affiliation:
Centre is for Mountain Agriculture, University of Innsbruck, Technikerstrasse 13, 6020 Innsbruck, Austria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Beetles / genetics*,  physiology*
DNA / analysis*
Feeding Behavior / physiology*
Food Chain*
Gastrointestinal Contents
Pest Control
Polymerase Chain Reaction / methods
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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