| Detecting oxygen consumption in the proximity of Saccharomyces cerevisiae cells using self-assembled fluorescent nanosensors. | |
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MedLine Citation:
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PMID: 16878334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a strategy for the preparation and self-assembly of fluorescent nanosensors onto Saccharomyces cerevisiae cell surfaces for dynamically measuring oxygen concentration in the proximity of living cells. Amine functionalized polystyrene nanobeads were impregnated with an oxygen-sensitive ruthenium(II) complex and the beads' surface was coated with polyethylenimine. The resulting nanosensors were assembled on individual S. cerevisiae cells in a controlled manner at physiological pH for continuously monitoring oxygen consumption. This approach exemplifies a general scheme for assembling fluorescent nanosensors on cells for the non-invasive, reversible, and real-time measurement of other physiologically relevant processes, such as the efflux of protons and carbon dioxide, or the influx of glucose. |
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Authors:
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Yina Kuang; David R Walt |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 96 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-01-01 Completed Date: 2007-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 318-25 Citation Subset: IM |
Copyright Information:
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(c) 2006 Wiley Periodicals, Inc. |
Affiliation:
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Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescent Dyes* Nanotechnology / methods* Oxygen / analysis*, metabolism Ruthenium* / chemistry Saccharomyces cerevisiae / metabolism* Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 7440-18-8/Ruthenium; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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