| Detecting natural abundance carbon signal of NAA metabolite within 12-cm3 localized volume of human brain using 1H-[13C] NMR spectroscopy. | |
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MedLine Citation:
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PMID: 9702699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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NMR spectroscopy has been applied extensively to study metabolism noninvasively in the human brain and other tissues. However, it usually suffers from poor signal-to-noise ratio due to low NMR sensitivity and low metabolite concentrations. In this study, the technique of proton-observe-carbon-edited (POCE) NMR spectroscopy combined with a single-shot localization sequence was used to detect the natural abundance carbon signal of the amino acid N-acetyl aspartate from a 12-cm3 localized volume in the occipital lobe of humans at 4 T. The results suggest that NMR spectroscopy is sensitive enough to detect signals from low concentration metabolites (< 60 nmol/g) from small volumes in the human brain within several minutes of data acquisition. This reveals that in vivo NMR spectroscopy is a promising technique for detecting small metabolite changes and low traces of 13C isotopic labeling for dynamic metabolism studies aimed at investigating physiological and pathological questions. |
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Authors:
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W Chen; G Adriany; X H Zhu; R Gruetter; K Ugurbil |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 40 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-11-24 Completed Date: 1998-11-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 180-4 Citation Subset: IM |
Affiliation:
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Radiology Department, University of Minnesota, School of Medicine, Minneapolis 55455, USA. wei@geronimo.drad.umn.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspartic Acid
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analogs & derivatives*,
metabolism Brain / metabolism* Carbon / metabolism* Humans Magnetic Resonance Spectroscopy / instrumentation* Sensitivity and Specificity Signal Processing, Computer-Assisted / instrumentation* |
| Grant Support | |
ID/Acronym/Agency:
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RR08079/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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56-84-8/Aspartic Acid; 7440-44-0/Carbon; 997-55-7/N-acetylaspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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