Document Detail


Detecting food- and waterborne viruses by surface-enhanced Raman spectroscopy.
MedLine Citation:
PMID:  20629888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Food- and waterborne viruses pose serious health risks to humans and were associated with many outbreaks worldwide. Rapid, accurate, and nondestructive methods for detection of viruses are of great importance to protect public health. In this study, surface-enhanced Raman spectroscopy (SERS) coupled with gold SERS-active substrates was used to detect and discriminate 7 food- and waterborne viruses, including norovirus, adenovirus, parvovirus, rotavirus, coronavirus, paramyxovirus, and herpersvirus. Virus samples were purified and dialyzed in phosphate buffered saline (8 to 9 log PFU/mL) and then further diluted in deionized water for SERS measurement. After capturing the characteristic SERS spectral patterns, multivariate statistical analyses, including soft independent modeling of class analogy (SIMCA) and principal component analysis (PCA), were employed to analyze SERS spectral data for characterization and identification of viruses. The results show that SIMCA was able to differentiate viruses with and without envelope with >95% of classification accuracy, while PCA presented clear spectral data segregations between different virus strains. The virus detection limit by SERS using gold substrates reached a titer of 10(2).
Authors:
Cui Fan; Zhiqiang Hu; Lela K Riley; Gregory A Purdy; Azlin Mustapha; Mengshi Lin
Related Documents :
2006648 - An outbreak of shigellosis at an outdoor music festival.
16672468 - Quantitative microbial risk assessment models for consumption of raw vegetables irrigat...
1822878 - Food-borne parasitic zoonoses in the philippines.
20807108 - Surveillance for foodborne illness outbreaks in qassim, saudi arabia, 2006.
18387108 - Preparation of low-fat uptake doughnut by dry particle coating technique.
17220488 - Work conditions, job preparation, and placement strategies for food-animal veterinarians.
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food science     Volume:  75     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-15     Completed Date:  2010-10-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M302-7     Citation Subset:  IM    
Affiliation:
Food Science Program, Div. of Food Systems & Bioengineering, Univ. of Missouri, Columbia, MO 65211, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenoviridae
Coronavirus
Feasibility Studies
Food Microbiology*
Herpesviridae
Norovirus
Parvovirus
Principal Component Analysis
Rotavirus
Sendai virus
Spectrum Analysis, Raman / methods*
Surface Properties
Viruses / isolation & purification*
Water Microbiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibition of shiga toxin 2 (stx2) in apple juices and its resistance to pasteurization.
Next Document:  Control of dongchimi fermentation with chitosan deacetylated by alkali treatment to prevent over-rip...