Document Detail

Detecting fetomaternal hemorrhage: a comparison of five methods.
MedLine Citation:
PMID:  1850569     Owner:  NLM     Status:  MEDLINE    
Appropriate postpartum administration of Rh immune globulin relies on sensitive detection and accurate quantitation of fetomaternal hemorrhage (FMH). Recently, the microscopic Du test (micro Du) enhanced with polyethylene glycol (PEG Du) and flow cytometry (FC) have been advocated for this purpose. Three qualitative methods (micro Du, rosette test, and PEG Du) and two quantitative methods (acid elution and FC) for assessing FMH were evaluated with particular attention given to PEG Du and FC. In vitro studies comprised 10 series of dilutions of D+ cord cells in D- adult cells to yield D+ cell concentrations of 0.06, 0.12, 0.25, 0.50, 0.75, 1.0, and 2.0 percent. Additionally, 26 postpartum samples were tested. Of the qualitative techniques, the micro Du test was the least sensitive with 20 percent false-negative results occurring at 0.5 percent fetal cells. The PEG Du test was only slightly more sensitive and offered no clinical advantage. The rosette test was the most sensitive, consistently detecting fetal cells at concentrations of 0.25 percent or greater. FC and acid elution showed similar results, with good correlation obtained between measured and expected quantities of fetal cells (r = 0.99 and 0.96, respectively). One of 26 postpartum samples was positive by all screening techniques; acid elution and FC detected 0.3-percent concentrations of fetal cells and 0.17-percent concentrations of D+ cells, respectively. Although acid elution is a more commonly used method for quantitating FMH, FC offers an acceptable alternative that is capable of analyzing large numbers of cells with objectivity and reproducibility.
K M Bayliss; B D Kueck; S T Johnson; J T Fueger; P W McFadden; D Mikulski; J L Gottschall
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Publication Detail:
Type:  Comment; Comparative Study; Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  31     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-05-30     Completed Date:  1991-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  303-7     Citation Subset:  IM    
Department of Pathology, Medical College of Wisconsin, Milwaukee.
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MeSH Terms
Coombs' Test
Fetomaternal Transfusion / complications,  diagnosis*
Flow Cytometry
Hydrogen-Ion Concentration
Immunization, Passive
Immunoglobulins / administration & dosage
Polyethylene Glycols
Rh Isoimmunization / etiology,  prevention & control*
Rh-Hr Blood-Group System / immunology
Rho(D) Immune Globulin
Rosette Formation
Reg. No./Substance:
0/Immunoglobulins; 0/Polyethylene Glycols; 0/Rh-Hr Blood-Group System; 0/Rho(D) Immune Globulin
Comment On:
Transfusion. 1991 May;31(4):288-9   [PMID:  1850567 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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