Document Detail


Detecting effects of low levels of cytochalasin B in 3T3 fibroblast cultures by analysis of electrical noise obtained from cellular micromotion.
MedLine Citation:
PMID:  19026529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We performed micromotion experiments using electric cell-substrate impedance sensing (ECIS) on a confluent layer of 3T3 fibroblasts exposed to different low levels of the toxin cytochalasin B. This toxin is know to affect actin polymerization and to disrupt cytoskeletal structure and function in cells, changing the morphology of confluent cell cultures and altering the nature of the cellular micromotion, which is measured by ECIS as changes in impedance. By looking at several measures to characterize the long- and short-term correlations in the noise of the impedance time series, we are able to detect the effects of the toxin at concentrations down to 1 microM; there are intriguing hints that the effects may be discernible at levels as low as 0.1 microM. These measures include the power spectrum, the Hurst and detrended-fluctuation-analysis exponents, and the first zero and first 1/e crossings of the autocorrelation function. While most published work with ECIS uses only average impedance values, we demonstrate that noise analysis provides a more sensitive probe.
Authors:
Douglas C Lovelady; Jennifer Friedman; Sonali Patel; David A Rabson; Chun-Min Lo
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2008-10-17
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  24     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-24     Completed Date:  2009-05-26     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2250-4     Citation Subset:  IM    
Affiliation:
Department of Physics, University of South Florida, Tampa, FL 33620, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Artifacts*
Biosensing Techniques / instrumentation*,  methods
Cytochalasin B / analysis*
Electrochemistry / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis
Mice
Motion
Reproducibility of Results
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
1R03CA123621-01A1/CA/NCI NIH HHS; R03 CA123621-01A1/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
14930-96-2/Cytochalasin B
Comments/Corrections

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