Document Detail


Detecting biochemical changes in the rodent cervix during pregnancy using Raman spectroscopy.
MedLine Citation:
PMID:  22411265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The goal of this research is to determine whether Raman spectroscopy (RS), an optical method that probes the vibrational modes of tissue components, can be used in vivo to study changes in the mouse cervix during pregnancy. If successful, such a tool could be used to detect cervical changes due to pregnancy, both normal and abnormal, in animal models and humans. For this study, Raman spectra were acquired before, during and after a 19-day mouse gestational period. In some cases, after Raman data was obtained, cervices were excised for structural testing and histological staining for collagen and smooth muscle. Various peaks of the Raman spectra, such as the areas corresponding to fatty acid content and collagen organization, changed as the cervix became softer in preparation for labor and delivery. These findings correspond to the increase in compliance of the tissue and the collagen disorganization visualized with the histological staining. The results of this study suggest that non-invasive RS can be used to study cervical changes during pregnancy, labor and delivery and can possibly predict preterm delivery before overt clinical manifestations, potentially lead to more effective preventive and therapeutic interventions.
Authors:
Elizabeth Vargis; Naoko Brown; Kent Williams; Ayman Al-Hendy; Bibhash C Paria; Jeff Reese; Anita Mahadevan-Jansen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-13
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  40     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-06-28     Completed Date:  2012-10-31     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1814-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cervix Uteri / metabolism*
Collagen / metabolism*
Fatty Acids / metabolism*
Female
Male
Mice
Obstetric Labor, Premature / diagnosis,  metabolism
Pregnancy / metabolism*
Spectrum Analysis, Raman*
Grant Support
ID/Acronym/Agency:
HD 044741/HD/NICHD NIH HHS; R01 HD044741/HD/NICHD NIH HHS; R01-CA-095405/CA/NCI NIH HHS; T32-HL7751-15/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 9007-34-5/Collagen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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