| Detailed structural and quantitative analysis reveals the spatial organization of the cell walls of in vivo grown mycobacterium leprae and in vitro grown M. tuberculosis. | |
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MedLine Citation:
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PMID: 21555513 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The cell wall of mycobacteria consists of an outer membrane, analogous to that of gram-negative bacteria, attached to peptidoglycan (PG) via a connecting polysaccharide arabinogalactan (AG). Although the primary structure of these components is fairly well deciphered, issues such as the coverage of the PG layer by covalently attached mycolates in the outer membrane and the spatial details of the mycolic acid attachment to the arabinan have remained unknown. It is also not understood how these components work together to lead to the classical acid-fast staining of mycobacteria. Since the majority of Mycobacterium tuberculosis bacteria in established experimental animal infections are acid fast negative, clearly cell wall changes are occuring. To address both the spatial properties of mycobacterial cell walls and to begin to study the differences between bacteria grown in animals and cultures, the cell walls of Mycobacterium leprae grown in armadillos was characterized and compared to that of M. tuberculosis grown in culture. Most fundamentally, it was determined that the cell wall of M. leprae contained significantly more mycolic acids attached to PG than that of in vitro grown M. tuberculosis (mycolate:PG ratios of 21:10 versus 16:10 respectively). In keeping with this difference, more arabinogalactan (AG) molecules, linking the mycolate acids to PG were found. Differences in the structures of the AG were also found; the AG of M. leprae is smaller than that of M. tuberculosis, although the same basic structural motifs are retained. |
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Authors:
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Suresh Bhamidi; Michael S Scherman; Victoria Jones; Dean C Crick; John T Belisle; Patrick J Brennan; Michael R McNeil |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-9 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Colorado State University, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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