| Detailed molecular delineation of 13q14.3 loss in B-cell chronic lymphocytic leukemia. | |
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MedLine Citation:
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PMID: 9454769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A region of chromosome 13q14.3, telomeric to the Retinoblastoma gene RB-1 is frequently deleted in patients with B-cell chronic lymphocytic leukemia (B-CLL). A cosmid and P1-derived artificial chromosome (PAC) contig spanning over 600 kb has been constructed, which encompasses this locus. The contig clones have been used to order a number of markers along the minimally deleted region and to localize a series of CpG islands corresponding to possible candidate genes. A novel polymorphic dinucleotide repeat, 6E3.2, present in one of the ordered cosmid clones has been isolated for use in deletion mapping studies of patient DNA. Leukemic samples from 229 CLL patients have been screened for loss of heterozygosity using microsatellite markers and analyzed for hemizygous and homozygous deletions by Southern blot techniques using genomic probes selected from cosmids across the region. Hemizygous deletions were found in 31% of cases with an additional 10% showing homozygous loss. The use of these probes has defined the commonly deleted area to less than 130 kb, centromeric to the locus D13S272. |
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Authors:
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M M Corcoran; O Rasool; Y Liu; A Iyengar; D Grander; R E Ibbotson; M Merup; X Wu; V Brodyansky; A C Gardiner; G Juliusson; R M Chapman; G Ivanova; M Tiller; G Gahrton; N Yankovsky; E Zabarovsky; D G Oscier; S Einhorn |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Blood Volume: 91 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-03-03 Completed Date: 1998-03-03 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1382-90 Citation Subset: AIM; IM |
Affiliation:
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Molecular Biology Laboratory, Royal Bournemouth General Hospital, Bournemouth, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 13* Genetic Markers Genome, Human Humans Leukemia, Lymphocytic, Chronic, B-Cell / genetics* Retinoblastoma Protein / genetics Sequence Deletion* |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 0/Retinoblastoma Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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