Document Detail

Detailed analysis of reactive oxygen species induced by visible light in various cell types.
MedLine Citation:
PMID:  20662023     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVE: Light in the visible and near infrared region stimulates various cellular processes, and thus has been used for therapeutic purposes. One of the proposed mechanisms is based on cellular production of reactive oxygen species (ROS) in response to illumination. In the present study, we followed visible light (VL)-induced hydroxyl radicals in various cell types and cellular sites using the electron paramagnetic resonance (EPR) spin-trapping technique.
MATERIALS AND METHODS: Fibroblasts, sperm cells, cardiomyocytes, and skeletal muscle cells were irradiated with broadband (400-800 nm) VL. To detect ROS, the EPR spin-trapping technique coupled with the spin-traps 5,5-dimethyl pyrroline-N-oxide (DMPO) or 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO) were used. To investigate the cellular sites of ROS formation, the cell-permeable molecule, isopropanol, or the nonpermeable proteins, bovine serum albumin (BSA) and superoxide dismutase (SOD), were introduced to the cells before irradiation. ROS production in mitochondria was measured using the fluorescent probe, MitoTracker Red (MTR).
RESULTS AND CONCLUSIONS: The concentration of .OH increased both with illumination time and with cell concentration, and decreased when N(2) was bubbled into the cell culture, suggesting that VL initiates a photochemical reaction via endogenous photosensitizers. VL was found to stimulate ROS generation both in membrane and cytoplasm. In addition, fluorescent measurments confirmed the mitochondria to be target for light-cell interaction. The findings support the hypothesis that ROS are generated in various cellular sites following light illumination.
Ronit Lavi; Asher Shainberg; Vladimir Shneyvays; Elicheva Hochauser; Ahuva Isaac; Tova Zinman; Harry Friedmann; Rachel Lubart
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  42     ISSN:  1096-9101     ISO Abbreviation:  Lasers Surg Med     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-27     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  473-80     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Department of Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel.
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MeSH Terms
Cell Line
Cell Membrane / metabolism
Cyclic N-Oxides
Cytoplasm / metabolism
Electron Spin Resonance Spectroscopy
Fibroblasts / metabolism*
Hydroxyl Radical / metabolism
Mitochondria / metabolism
Muscle, Skeletal / cytology*,  metabolism
Myocytes, Cardiac / metabolism*
Reactive Oxygen Species / metabolism*
Spermatozoa / metabolism*
Reg. No./Substance:
0/(diethoxyphosphoryl)-5-methylpyrroline N-oxide; 0/Cyclic N-Oxides; 0/Pyrroles; 0/Reactive Oxygen Species; 3317-61-1/5,5-dimethyl-1-pyrroline-1-oxide; 3352-57-6/Hydroxyl Radical

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