Document Detail

Detachment-associated changes in lipid rafts of senescent human fibroblasts.
MedLine Citation:
PMID:  16546133     Owner:  NLM     Status:  MEDLINE    
We characterized the effects of in vitro cellular aging on constituents of lipid rafts in human diploid fibroblasts, TIG-1. Cholesterol recovery from lipid rafts of senescent cells was decreased by the detaching treatment, while the decrease was far less obvious in young cells. A probe that binds selectively to cholesterol in lipid rafts revealed that the amount of lipid rafts on the cell surface decreased in senescent cells upon cell detachment. Accompanying this change was the release of the raft-associated molecules caveolin and Fyn from lipid rafts upon cell detachment, suggesting a detachment-associated disorganization of lipid rafts in senescent cells. In addition, our observations showing differential sensitivities of lipid rafts from young and senescent cells to detaching treatment indicate a caution in how to detach cells. Particular attention needs to be paid to interpreting the results when lipid rafts are prepared from mechanically detached cells under detergent-free conditions.
Mitsushi Inomata; Yukiko Shimada; Masami Hayashi; Hiroshi Kondo; Yoshiko Ohno-Iwashita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-10
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  343     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-03-27     Completed Date:  2006-05-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  489-95     Citation Subset:  IM    
Biomembrane Research Group, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan.
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MeSH Terms
Cell Aging / physiology*
Cell Line
Fibroblasts / metabolism*,  ultrastructure*
Membrane Microdomains / metabolism*,  ultrastructure*
Membrane Proteins / metabolism*
Reg. No./Substance:
0/Membrane Proteins

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