| Destruction of erythroleukemia, myelocytic leukemia and Burkitt lymphoma cells by photoactivated protoporphyrin. | |
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MedLine Citation:
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PMID: 6941938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of protoporphyrin on erythroid, myeloid and lymphoid leukemic cells and their destruction induced by the photoactivated porphyrin was studied. Friend erythroleukemic cells (FL) and myelocytic leukemic cells (ML) accumulated protoporphyrin in a cap or patch-like pattern observed by fluorescence microscopy. Photoactivated protoporphyrin induced the appearance of "holes" on the cell membrane demonstrated by scanning electron microscopy. On the other hand, Burkitt lymphoma (BL) and mastocytoma (MS) cells accumulated porphyrin intracellularly around the nuclear envelope and as circular profiles, respectively. Photoactivated protoporphyrin induced development of multiple blebs on the cell membrane, and even complete cell destruction. Cytotoxicity of protoporphyrin at short-term incubation periods was determined by [3H]thymidine and [3H]uridine incorporation. Protoporphyrin, unexposed to light, reduced the incorporation of both precursors only to a moderate extent. On the other hand, porphyrin-treated cells exposed to light showed complete inhibition of RNA and DNA synthesis. Long-term exposure of ML and BL cells to porphyrin in the dark induced a nearly 50% inhibition of RNA and DNA synthesis. Although the cytotoxic effect of protoporphyrin in the dark was lower than that of photoactivated porphyrin, this may possess a potential activity in vivo even without illumination. |
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Authors:
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Z Malik; M Djaldetti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 26 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1980 Oct |
Date Detail:
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Created Date: 1981-09-25 Completed Date: 1981-09-25 Revised Date: 2007-07-24 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: DENMARK |
Other Details:
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Languages: eng Pagination: 495-500 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Burkitt Lymphoma / pathology* Cell Line DNA, Neoplasm / biosynthesis Leukemia, Erythroblastic, Acute / pathology* Leukemia, Myeloid / pathology* Photic Stimulation* Porphyrins / pharmacology* Protoporphyrins / metabolism, pharmacology* RNA, Neoplasm / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Porphyrins; 0/Protoporphyrins; 0/RNA, Neoplasm |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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