| Destruction of cholera toxin receptor on HeLa cell membrane using microbial endoglycoceramidase. | |
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MedLine Citation:
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PMID: 8638937 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sensitivity of HeLa cells to cholera toxin decreased by Corynebacterium sp. endoglycoceramidase treatment. This endo-enzyme destroyed the cholera toxin receptor, ganglioside G(M1), on the cell surface membrane by liberating intact oligosaccharide from it, which was confirmed by the decrease of intracellular cAMP accumulation and the results of the analysis of released oligosaccharide with a combination of pyridylamination method and HPLC. Fluorescence microscopy using the immunofluorescence method revealed that the amount of cholera toxin attached to the cells decreased in endoglycoceramidase-treated cells. The enzyme acted on cellular glycosphingolipids without addition of any activator protein which is required by other similar enzymes. Corynebacterium endoglycoceramidase is a useful tool to elucidate the function of glycosphingolipids on the cell surface in situ. |
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Authors:
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K Yamamoto; T Nagano; H Kumagai; Y Okamoto; S Otani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 328 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-07-10 Completed Date: 1996-07-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 51-6 Citation Subset: IM |
Affiliation:
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Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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metabolism* Cholera Toxin / metabolism* Chromatography, High Pressure Liquid Corynebacterium / enzymology Cyclic AMP / metabolism Fluorescent Antibody Technique, Indirect G(M1) Ganglioside / metabolism* Glycoside Hydrolases / metabolism* Hela Cells Humans Oligosaccharides / metabolism Protein Binding Receptors, Cell Surface / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; 0/Receptors, Cell Surface; 0/choleragen receptor; 37758-47-7/G(M1) Ganglioside; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.123/endoglycoceramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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