| Desolvation process and surface characterisation of protein nanoparticles. | |
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MedLine Citation:
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PMID: 10601688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of the present study was to characterise and optimise the desolvation process of human serum albumin (HSA) for the preparation of nanoparticles and to characterise the resulting colloidal system. Following the desolvation of the protein, the resulting nanoparticles were stabilised by the addition of varying amounts of glutaraldehyde or by heat denaturation. The particle size, zeta potential, and the number of available amino groups on the surface of the nanoparticles were determined. The amino groups were quantified by a spectrophotometric method using 2,4, 6-trinitrobenzenesulfonic acid (TNBS). The results indicated that the particle size depended mainly on the amount of desolvating agent added, but not on the amount of cross-linker or the kind of cross-linking procedure. Increasing amounts of glutaraldehyde reduced the number of amino groups on the surface of HSA nanoparticles and also decreased the zeta potential of the carrier system. The temperature and heat denaturation time only had an influence on the stability of the nanoparticles but not on the amount of amino groups or the particle size. It was shown that heat denatured HSA nanoparticles possessed the greatest number of amino groups on their surface. Additional experiments for the characterisation of gelatin A and B nanoparticles were performed. |
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Authors:
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C Weber; C Coester; J Kreuter; K Langer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 194 ISSN: 0378-5173 ISO Abbreviation: Int J Pharm Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-03-16 Completed Date: 2000-03-16 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 91-102 Citation Subset: IM |
Affiliation:
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Institut f]ur Pharmazeutische Technologie, Biozentrum Niederursel, Johann Wolfgang Goethe-Universit]at, Marie-Curie-Strasse 9, D-60439, Frankfurt am Main, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Colloids
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chemistry Cross-Linking Reagents / chemistry Drug Carriers Gelatin / chemistry Glutaral / chemistry Humans Kinetics Particle Size Protein Denaturation Serum Albumin / chemistry* Solubility Spectrophotometry / methods Surface Properties Trinitrobenzenesulfonic Acid / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Colloids; 0/Cross-Linking Reagents; 0/Drug Carriers; 0/Serum Albumin; 111-30-8/Glutaral; 2508-19-2/Trinitrobenzenesulfonic Acid; 9000-70-8/Gelatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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