|Desmoglein 2 mutant mice develop cardiac fibrosis and dilation.|
|Jump to Full Text|
|PMID: 21455723 Owner: NLM Status: MEDLINE|
|Desmosomes are cell-cell adhesion sites and part of the intercalated discs, which couple adjacent cardiomyocytes. The connection is formed by the extracellular domains of desmosomal cadherins that are also linked to the cytoskeleton on the cytoplasmic side. To examine the contribution of the desmosomal cadherin desmoglein 2 to cardiomyocyte adhesion and cardiac function, mutant mice were prepared lacking a part of the extracellular adhesive domain of desmoglein 2. Most live born mutant mice presented normal overall cardiac morphology at 2 weeks. Some animals, however, displayed extensive fibrotic lesions. Later on, mutants developed ventricular dilation leading to cardiac insufficiency and eventually premature death. Upon histological examination, cardiomyocyte death by calcifying necrosis and replacement by fibrous tissue were observed. Fibrotic lesions were highly proliferative in 2-week-old mutants, whereas the fibrotic lesions of older mutants showed little proliferation indicating the completion of local muscle replacement by scar tissue. Disease progression correlated with increased mRNA expression of c-myc, ANF, BNF, CTGF and GDF15, which are markers for cardiac stress, remodeling and heart failure. Taken together, the desmoglein 2-mutant mice display features of dilative cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy, an inherited human heart disease with pronounced fibrosis and ventricular arrhythmias that has been linked to mutations in desmosomal proteins including desmoglein 2.|
|Claudia A Krusche; Bastian Holthöfer; Valérie Hofe; Annette M van de Sandt; Leonid Eshkind; Ernesto Bockamp; Marc W Merx; Sebastian Kant; Reinhard Windoffer; Rudolf E Leube|
Related Documents :
|1950993 - Isolated significant left main coronary artery stenosis: angiographic, hemodynamic, and...
3486703 - Left ventricular function in coronary disease: serial studies in the absence of myocard...
25236513 - Comprehensive prognosis assessment by cmr imaging after st-segment elevation myocardial...
|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-04-01|
|Title: Basic research in cardiology Volume: 106 ISSN: 1435-1803 ISO Abbreviation: Basic Res. Cardiol. Publication Date: 2011 Jun|
|Created Date: 2011-06-01 Completed Date: 2011-10-03 Revised Date: 2013-06-30|
Medline Journal Info:
|Nlm Unique ID: 0360342 Medline TA: Basic Res Cardiol Country: Germany|
|Languages: eng Pagination: 617-33 Citation Subset: IM|
|Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Wendlingweg 2, Aachen, Germany. email@example.com|
|APA/MLA Format Download EndNote Download BibTex|
Cardiomegaly / etiology
Desmoglein 2 / physiology*
Growth Differentiation Factor 15 / genetics
Mice, Inbred C57BL
Myocardium / pathology*
|0/Desmoglein 2; 0/Dsg2 protein, mouse; 0/Gdf15 protein, mouse; 0/Growth Differentiation Factor 15|
Journal ID (nlm-ta): Basic Res Cardiol
Publisher: Springer-Verlag, Berlin/Heidelberg
© Springer-Verlag 2011
Received Day: 18 Month: 8 Year: 2010
Revision Received Day: 9 Month: 3 Year: 2011
Accepted Day: 21 Month: 3 Year: 2011
Electronic publication date: Day: 1 Month: 4 Year: 2011
pmc-release publication date: Day: 1 Month: 4 Year: 2011
Print publication date: Month: 7 Year: 2011
Volume: 106 Issue: 4
First Page: 617 Last Page: 633
PubMed Id: 21455723
Publisher Id: 175
|Desmoglein 2 mutant mice develop cardiac fibrosis and dilation|
|Claudia A. Krusche1||
Address: +49-241-8080756 +49-241-8082508 firstname.lastname@example.org
|Annette M. van de Sandt2|
|Marc W. Merx2|
|Rudolf E. Leube1||
Address: +49-241-8089107 +49-241-8082508 email@example.com
1Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany
2Division of Cardiology, Angiology and Pneumology, Department of Medicine, University Hospital Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany
3Institute for Toxicology, Medical Centre of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, 55131 Mainz, Germany
The intercalated disc is a complex cell–cell attachment site mediating mechanical and electrical coupling between cardiomyocytes. It consists of adherens junctions, which facilitate the attachment of the actin filament-containing contractile apparatus and desmosomes, which serve as anchorage sites for desmin intermediate filaments. Desmosomes are symmetric multiprotein assemblies with their halves interconnected to transmit forces between adjacent cells (reviews in [19, 21, 28]). At the core are calcium-dependent adhesion proteins of the cadherin type that are grouped into two families, the desmocollins and desmogleins. In the heart, desmoglein 2 (Dsg2) and desmocollin 2 are by far the most prominent, if not sole desmosomal cadherins . These polypeptides interact in cis and trans forming homo- and heteropolymers via their extracellular domains EC1–EC4, the configuration of which is influenced by calcium ion binding [13, 55, 59, 60]. It is assumed that the insertion of tryptophane 2 into a hydrophobic pocket of the EC1 domain and EC1–EC2 interactions are important for multimerization and engagement in zipper-like transcellular arrangements (e.g.,  for C-cadherin), which is supported by ultrastructural analyses [1, 23] and antibody localization studies . Desmosomal cadherins are connected to cytoplasmic linker molecules, which are localized to the desmosomal plaque. These linkers include plakoglobin and the cell-type specifically expressed plakophilins (cf. [19, 21, 28]). In the case of plakoglobin, direct desmosomal cadherin binding sites have been identified (e.g., ). Desmoplakin, a member of the plakin cytolinker family, is another major component of the desmosomal plaque. It binds to plakophilins and mediates intermediate filament attachment to desmosomal junctions [19, 21, 28].
Mutations of the genes encoding Dsg2, desmocollin 2, plakoglobin, plakophilin 2 and desmoplakin are found in approximately 50% of patients with arrhythmogenic right ventricular cardiomyopathy (ARVC) [2, 24, 57]. Thus, ARVC is considered to be a disease of the desmosome, although other factors are also implicated [5, 20, 24, 39, 51, 58]. ARVC occurs at a frequency of 1:1,000 to 1:5,000 [3, 9] and leads to heart failure or sudden cardiac death in young adults. Excessive dilation, predominantly of the right ventricle, progressive fibrofatty replacement of cardiomyocytes and arrhythmias are the main features of the disease (recent reviews [24, 57, 61]). Especially in patients carrying mutations in the desmoplakin and DSG2 genes, the left ventricle is also severely affected . In addition, the V55M missense mutation of the DSG2 gene predisposes to dilative cardiomyopathy .
To analyze the impact of the adhesive extracellular Dsg2 domains in demososme-related heart function, mouse strains were established lacking exons 4–6 of the DSG2 gene, which code for major parts of the EC1/EC2 domains. Homozygous mutant mice presented heart disease with necrotic cell death of cardiomyocytes, cardiac fibrosis and dilation of heart chambers leading to cardiac dysfunction.
All synthetic oligonucleotides are listed in Table 1. A targeting construct was prepared to conditionally delete exons 3 and 4 of murine DSG2. First, the 5′-homology region between introns 3 and 6 was subcloned as a 5,483 bp HindIII-fragment from phage λMg1 (cf. ) into pBluescript KS(+) resulting in clone 3001. A 5′-loxP-site was introduced by insertion of annealed oligomers loxP-f/loxP-r into the NheI-site of intron 3 (clone 3007). A second XhoI-site was then introduced by ligating annealed linkers Eco-Xho-Bst-f/Eco-Xho-Bst-r into the EcoRI- and BstXI-sites of clone 3007 (clone 3010). For cloning the 3′-arm, pBluescript II_SK(+) was first modified by inserting annealed linkers 03-51pBS2SK-EI+Sph-a/03-51pBS2SK-EI+Sph-b into the EcoRI-site without reconstituting it (clone 3000). Then, the ~4.6 kb HindIII-fragment of λMg1 was cloned into clone 3000 producing clone 3004. To afford the usage of a 5′-external probe that had been used previously for Southern blot analyses , the SphI-site at the 3′-end of clone 3004 was removed by blunt-end ligation after digestion with Bst1107I and SmaI resulting in clone 3008. A construct with the neomycin-resistance cassette (NFL) under control of the phosphoglycerate kinase promoter and flanked by two directed FRT-sites and an additional loxP-site at the 3′-end was provided by Dr. Gale Martin. It was used for PCR-amplification with primers 05-251-Eco-PGK-Neo-f and 05-252-Eco-PGK-Neo-r, which introduced EcoRI-sites at either end. The EcoRI-endowed DNA was thereafter ligated into the unique EcoRI-site of exon 6 in clone 3008, thereby creating clone 3009. Clones 3009 and 3010 were then digested with XhoI and the appropriate genomic fragments were ligated rendering the final targeting construct (clone 3011).
NotI-linearized clone 3011 was transfected into embryonal stem cell strain W9.5  by electroporation (single pulse, 500 μF, 0.24 kV). G418-resistant colonies were isolated and subjected to Southern blot analyses. To this end, EcoRV-digested and gel electrophoretically separated DNA was blotted onto nylon membranes and hybridized with two external and one internal (neomycin) probes that were generated by PCR. Primers 03-66_5ext_for/03-67_5ext_rev were used for the 5′-external probe, primers 03-54_3ext_for/03-72_3ext_rev2 for the 3′-external probe, and primers 03-70_NeoProbe_for/03-71_NeoProbe_rev for the internal neomycin probe (Table 1). The PCR products were labeled with 32P-dATP by random primed oligo labeling (Stratagene). Cell clones with correct recombination were used to prepare transgenic animals (cf. ). Chimeric males were backcrossed with C57BL/6J females and the dark-haired offspring was genotyped. To remove the NFL-cassette, transgenic animals were crossed with FLPe deleter mice . FLP-mediated recombination was assayed by PCR (primers 03-57b and 03-58_3′rev; Table 1) and Southern blot analyses. Animals with correctly recombined alleles were intercrossed with the constitutively Cre-expressing “ActCre” strain FVB/N-TgN(ACTB-Cre)2Mrt from Jackson Laboratories  to generate constitutively mutant animals.
For experimental analyses, heterozygous animals were crossed to produce wild-type and heterozygous littermate controls. Unfortunately, mutant offspring was often absent because of the small average litter size of six to eight animals and the approximately 70% reduction of mutant animals due to intrauterine lethality. As an alternative, heterozygous females were crossed with homozygous males, which was more efficient but lacked wild-type controls. Consequently, in the experiments, age-matched controls had to be used from parallel breedings. Analyzed mice still possess a mixed genetic background. To reduce genetic variability, mutant mice were backcrossed with C57BL/6J mice.
The sequences of all primers are listed in Table 1. For analysis of DSG2 mRNA expression, RNA was isolated from snap frozen heart tissue (RNeasy-Kit, Qiagen, Hilden, Germany). As much as 0.5 μg of total RNA was transcribed with Omniscript reverse transcriptase (Qiagen) and 1/4 of the cDNA was used as template for secondary strand synthesis with primers 07-12-f and 07-13-r, generating fragments of either 210 bp (recombined) or 686 bp (wt). GAPDH was used as control (primers 05-31-GAPDH-f and 05-32-GAPDH-r).
For all other mRNA analyses, total RNA was isolated with the High Pure RNA Tissue Kit (Roche, Mannheim, Germany); 25 mg tissue was homogenized with a glass–Teflon homogenizer in 900 μl lysis-/binding buffer and subjected to extraction with phenol/chloroform/isoamyl alcohol (Applied Biosystems, Darmstadt, Germany). As much as 1 μg of the affinity column-purified RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with the oligo-(dT)15 primer.
Real-time PCR was performed with a LightCycler (Roche) using the LightCycler TaqMan Master Kit (Roche). Forward and reverse primers and corresponding TaqMan probes were selected with the help of the Universal ProbeLibrary Assay Design Center (Roche). They are listed in Table 1 as the following pairs HMBS-f/HMBS-r (UPL#79), ANF-f/ANF-r (UPL-#25), BNF-f/BNF-r (UPL#71), c-myc-f/c-myc-r (UPL#77), CTGF-f/CTGF-r (UPL#71), GDF15-f/GDF15-r (UPL#62), RGS2-f/RGS2-r (UPL#6), and βMHC-f/βMHC-r (UPL#85). PCR programs were operated by the LightCycler Software Version 3.5. cDNA (2 μl) prepared from 100 ng total RNA was used in each PCR with only water and 100 ng RNA as negative controls. HMBS was used as housekeeping control . Each tissue sample was examined in duplicate. To determine PCR efficiency, a coefficient file was created for each target cDNA and the housekeeping control. Quantitative analyses of cardiac target mRNA expression were performed with the RelQuant Software, Version 1.01 (Roche).
Hearts were fixed in PBS containing 4% formaldehyde, washed and embedded in paraffin; 8-μm thick tissue sections were stained with hematoxylin/eosin, Kossa and Heidenhein (azan).
For immunohistochemistry, 5-μm thick paraffin sections were prepared. The sections were rehydrated, heated in citrate buffer (pressure cooker, 121°C, 3 min), cooled down for 20 min and incubated with primary antibodies against the extracellular anchor and EC4 domains of murine Dsg2 1:500 or 1:1,000  and desmoplakin (1:1,000 or 1:2,000, Progen, Heidelberg, Germany) overnight at 4° C. After washing in 50 mM Tris/HCl, 0.3 M NaCl, 0.05% Tween 20, pH 7.5, the sections were incubated for 1 h at room temperature with the secondary antibodies (Alexa488-conjugated goat anti rabbit antibodies [1:500] and Cy3-conjugated affinity purified goat anti-guinea pig antibodies [1:400; both from Dianova, Hamburg, Germany]). After three washing steps, autofluorescence was quenched with 0.1% Sudan black in 70% ethanol (30 min). The sections were counterstained with DAPI. Fluorescence micrographs were recorded with an LSM710 DUO (Zeiss, Jena, Germany). For quantification, non-saturating dilution ranges were determined for the primary antibodies. Quantification of intercalated disc staining was done for 40 intercalated discs per animal using the ZEN 2009-Software (Zeiss, Jena, Germany). The ratio of Dsg2- to desmoplakin-fluorescence was calculated for each intercalated disc and the mean value of the ratio of all intercalated discs of each studied animal was determined.
Peroxidase reaction was used for detection of Ki67 (rabbit mAb, Medac, Wedel, Germany) and cleaved caspase 3 (Cell Signaling Technology, Danvers, MA). Bound primary antibodies were detected with the ZytoChemPlus HRP Polymer anti-Mouse/Rabbit Kit (Zytomed Systems, Berlin, Germany). Paraffin sections of 5-μm thickness were deparaffinized in xylene and rehydrated in a graded alcohol series. During rehydration, the activity of the endogenous peroxidase was blocked by treating sections with 3% H2O2 in 70% ethanol for 10 min in the dark. Antigen retrieval was achieved by incubating sections in 10 mM citrate buffer (pH 6.0) at 94°C for 40 min (Ki67) or in a pressure cooker for 3 min at 121°C (cleaved caspase-3). Cooled down sections were treated with blocking solution of the HRP Polymer Kit. Then, sections were incubated for 2 h at room temperature with the Ki67-antibody (1:200) or overnight at 4°C with the cleaved caspase 3-antibody (1:300). After subsequent washing, the sections were covered with the HRP-polymer for 30 min. The AEC Substrate Kit (Invitrogen, Karlsruhe, Germany) was applied to visualize the immunoreaction and sections were counterstained with hematoxylin. The percentage of Ki67-positive interstitial cells was counted by two independent and blinded observers (CAK and SK). The percentage of Ki67-positive interstitial cells was assessed in three to four randomly chosen areas of the right and left ventricular wall, as well as in the septum at a magnification of 400×.
Fresh tissue fragments were minced by extensive homogenization in extraction buffer I (10 mM Tris–HCl, pH 8, 2 mM MgCl2, 10 mM KCl, 0.1% SDS, 0.05% PMSF supplemented with one tablet Complete mini (Roche, Mannheim, Germany) per 10 ml buffer) at 4°C. After shaking for 1 h at 4°C, the homogenate was centrifuged at 17,000×g for 25 min at 4°C. The cytosolic supernatant fraction was removed and the pellet was washed three times with extraction buffer. The pellet was then dissolved by shaking in extraction buffer I supplemented with 2% SDS for 2 h at 4°C. After centrifugation (17,000×g, 25 min, 4°C), the supernatant was used for immunoblotting. Protein concentration was determined by Lowry assay (Roth, Karlsruhe, Germany). Proteins were separated by standard SDS polyacrylamide gel electrophoresis and blotted onto PVDF membranes. Antibodies against Dsg2 (rabbit polyclonal 1:1,000; ), β-actin (rabbit polyclonal, A2066, 1:4,000, Sigma, Steinheim, Germany), desmocollin 2 (goat; 1:200; Santa Cruz Biotechnology, Santa Cruz, USA) and desmoplakin (mouse; Progen, Heidelberg, Germany) were diluted in TBST supplemented with 5% BSA and incubated with the membrane overnight at 4°C. After three washes in TBST, the membrane was incubated for 1 h with HRP-coupled secondary antibodies from DAKO (diluted 1:3,000 in TBST containing 5% milk powder). The ECL system was used for HRP detection (GE Healthcare, Freiburg, Germany).
Cardiac images were acquired using a Vevo770 high-resolution ultrasound scanner with 30-MHz linear transducer (VisualSonics Inc.). Echocardiography was performed under slight mask anesthesia by an inhaled mixture of 1.5% (v/v) isoflurane and 100% oxygen. ECGs were obtained with built-in ECG electrode-contact pads. Body temperature was maintained at 37°C by a heating pad. All hair was removed from the chest using a chemical hair remover (Nair; Carter-Horner; Mississauga, Canada). Aquasonic 100 gel (Parker Laboratories, Hellendoorn, The Netherlands) from which air bubbles had been expelled was applied to the thorax surface to optimize the visibility of the cardiac chambers. Parasternal long-axis and short-axis views were acquired. Image data obtained from multiple cardiac cycles were retrospectively assembled into a representative image sequence of over 100 images per cardiac cycle using the ECG-based Kilohertz Visualization (EKV) capability of the Vevo770 scanner. The EKV images are synchronized with respect to the ECG signal. Left ventricular (LV) end-systolic and end-diastolic volumes (ESV and EDV) were calculated by identification of frames with maximal and minimal cross-sectional area and width. The system software employs a formula based on a cylindric-hemiellipsoid model . LV ejection fraction (LVEF), cardiac output (CO), stroke volume (SV) and fractional area change (FAC) were calculated from volume data. M-mode recordings were analyzed for wall thicknesses and chamber dimensions. Fractional shortening (FS) was assessed as a measure of left ventricular systolic function. The Doppler transducer was positioned over the apex (apical four-chamber view) for sampling of the mitral flow wavefrom at the tips of the mitral valves. LV diastolic function was evaluated from the E/A ratio of Doppler tracings, where E is the peak velocity of early diastolic ventricular filling, and A is the peak velocity of late filling associated with atrial contraction. A single ultrasound session ranged from 15 to 30 min per mouse.
Invasive hemodynamics were assessed using a 1.4F Millar pressure–volume catheter (SPR-839, Millar Instrument, Houston, Texas) placed into the left ventricle through the right carotid artery. After surgical instrumentation, data were analyzed with the IOX Software (EMKA) to calculate left ventricular developed pressure (LVDP), and the first derivatives of left intraventricular pressure (rate of pressure development + dP/dtmax and rate of pressure decrease − dP/dtmin). Cardiac contractility index was calculated as +dP/dtmax normalized for the respective LVDP. Relaxation index was calculated as the ratio of −dP/dtmin to respective LVDP . To assess the catecholamine responsiveness, cardiac variables were recorded under continuous norepinephrine administration (0.4 μg/kg/min, i.p.). For ECG measurements, four lead surface ECGs were recorded before and during norepinephrine administration. Therefore, ECG electrodes were attached to each limb. The resulting ECGs were analyzed with the IOX Software (EMKA). At the end of the experiments, mice were killed by cervical dislocation.
Results are presented as mean ± SD (echocardiography) or as mean ± SEM (all other assessments). In cases, in which two groups were compared (echocardiography and RT-PCR of 2-week-old mice), data were analyzed by the unpaired Student’s t test. P values <0.05 were considered to be statistically significant. To analyze the results of three experimental groups, one way ANOVA and post hoc Bonferroni’s multiple comparison tests were used.
A targeting construct was prepared for inducible deletion of exons 4–6 of murine DSG2 (Fig. 1a). This mutation does not disrupt the reading frame and encodes mutant Dsg2 (Dsg2mt) that lacks a substantial segment of the EC1–EC2 domains, which are believed to participate in homo- and heterophilic desmosomal cadherin interactions (Fig. 1b; cf. [28, 59]). Transgenic mice were prepared from embryonal stem cells after homologous recombination of the targeting construct. The neomycin-resistance cassette was then removed with the help of Flpe deleter mice (Fig. 1a) . The resulting DSG2loxP/loxP offspring presented no obvious phenotypic deficiency. To investigate the consequences of constitutive DSG2 mutation, DSG2loxP animals were crossed with transgenic mice producing Cre-recombinase under β-actin promoter control . This led to deletion of exons 4–6 of DSG2 in germ cells and subsequent transmission of the mutated allele to the offspring. In contrast to the previously described DSG2 null mutants lacking exons 7 and 8, all of which died around implantation , approximately a third of the DSG2mt/mt mice lacking exons 4–6 were born apparently healthy, whereas the rest died during intrauterine development. DSG2mt mRNA and Dsg2mt protein are produced in these mice (Fig. 1c, d). Furthermore, Dsg2mt is located in intercalated discs (Fig. 1e). Dsg2mt expression, however, is considerably lower than that of the wild type (Fig. 1d, e).
Echocardiography was performed in 15- to 26-week-old male animals (summary of all results in Table 2). DSG2mt/mt mice exhibited dilation of the left ventricle (increased diameters in M-mode in Fig. 2a, b) and an elevated end-diastolic volume (Table 2). Elevated end-diastolic volume indicated reduced ventricular compliance. In accordance, early left ventricular filling had a tendency to be slower in DSG2mt/mt mice (mitral valve E-wave peak), whereas late diastolic blood flow velocity across the mitral valve was significantly reduced (mitral valve A-wave peak). Consequently, the E-wave to A-wave (E/A) ratio was significantly increased in the mutants, indicating impaired ventricular filling. In addition, systolic left ventricular diameter and systolic left ventricular end volume were increased in mutants indicating altered systolic function. Furthermore, ejection fraction, cardiac output and fractional shortening were all reduced (Table 2). Heart rates were comparable between wild type and mutants. Hemodynamic parameters were invasively examined by micro-conductance catheter to complement non-invasive echocardiography (see Table 2). In agreement with the impaired systolic function observed by echocardiography, left ventricular developed pressure (LVDP), the maximal rate of left ventricular pressure change (dP/dtmax), a parameter of cardiac contractility and the contractility index  were decreased in DSG2mt/mt mice (Table 2). Subjecting animals to stress by treatment with norepinephrine demonstrated that mutant hearts were still responsive, resulting in increase of LVDP, dP/dtmax and contractility index, albeit not to the extent of their wild-type counterparts (Table 2). Reflecting diastolic dysfunction, left ventricular relaxation was significantly impaired with reduced −dP/dtmin and relaxation index. Under norepinephrine stimulation, the mutant mice developed ventricular arrhythmias, e.g., ventricular extrasystoles, as well as atrial arrhythmias and repolarization abnormalities (Fig. 2f). One of the mutant animals died during anesthesia. ECG alterations were not recorded in any of the age- and sex-matched wild-type animals in the presence of norepinephrine (Fig. 2e).
Anatomically, dilation of both ventricles and in some cases also dilated atria were readily apparent in dissected female and male mutants (Figs. 2d, 3a, b). Furthermore, heart weight was significantly increased (Table 2). In one case of sudden death, rupture of the right ventricle was detected. Anatomical, histological and clinical parameters did not change upon successive inbreeding and backcrossing against C57BL/6J (online resource 1 and 2; unpublished data).
In dissected hearts of mutant mice, fibrotic lesions of various size and different shapes, often in the form of striations, were frequently seen. In some instances, the entire ventricular wall was completely replaced by fibrotic scar tissue resulting in substantial wall thinning (e.g., Fig. 4b, d). Kossa-positive calcifications were often found in fibrotic lesions and scars (Fig. 3c). Furthermore, groups of cardiomyocytes embedded in fibrous tissue and cardiomyocytes with dysmorphic nuclei were noted (Fig. 3d). Histological analyses further revealed different degrees of fibrosis in both ventricular walls and the septum (Fig. 4b, d, f). Staining with azan and anti-vimentin antibodies further supported these findings (e.g., Fig. 4h, j). Extensive infiltrates of immune cells were not seen except for scattered immune cells in a few regions.
Next, proliferation of interstitial cells faraway from fibrotic lesions and proliferation within fibrotic lesions was studied at 2, 8 and 13 weeks by Ki67 immunohistochemistry (Fig. 5). No differences in interstitial cell proliferation were detectable between DSG2wt/wt, DSG2wt/mt and DSG2mt/mt at 2 weeks (Fig. 5a–c). At 8 weeks, the proliferation index of interstitial cells was elevated in all segments of the ventricular walls of DSG2mt/mt mice compared to DSG2wt/wt animals (P < 0.05; Fig. 5d–f, k, l). Then, at 13 weeks, the percentage of proliferating cells did not differ between mutant and wild-type animals anymore (Fig. 5g–i).
Contiguous fibrotic lesions were examined next. A subset of DSG2mt/mt mice presented extensive lesions with necrotic calcinosis already at 2 weeks (Fig. 6c, f). Furthermore, cells expressing activated caspase-3 were also observed indicating apoptotic cell death (Fig. 6i). Significantly elevated Ki67-staining was detected within these lesions (54.4 ± 5.6%; Fig. 5 m) in comparison to interstitial cells in areas away from the lesion or in age-matched DSG2wt/wt and DSG2wt/mt hearts (P < 0.0001). Of note, the number of proliferating cells within such lesions was significantly higher in 2-week-old than in 8- and 13-week-old animals (Fig. 5j, m–o). The cells within the lesions were rounded in young animals and spindle shaped in the older mice (compare, Fig. 5m–o).
The expression of well-established cardiac stress and heart failure markers [14, 16] was examined at 2, 8 and 13 weeks by real time RT-PCR to assess the consequences of DSG2 mutation at the molecular level. At 2 weeks, cardiac connective tissue growth factor (CTGF) mRNA was significantly upregulated. In addition, growth differentiation factor 15 (GDF15) and c-myc mRNA were increased (Fig. 7). When 2-week-old DSG2mt/mt mice were further subclassified by visual inspection into those without and those with fibrotic lesions, significantly elevated GDF15 mRNA expression was found in the hearts with fibrotic lesions (3.83 ± 1.42 vs. 0.18 ± 0.03 arbitrary units; Table 3). This suggests that GDF15 mRNA expression is an early indicator of disease progression.
At 8 and 13 weeks, CTGF, GDF15 and c-myc mRNA are all significantly upregulated in the mutant animals (Fig. 7). In addition, the mRNA of atrial natriuretic peptide (ANF) and B-type natriuretic factor (BNF), which were not any different from the wild type at 2 weeks, were also significantly increased in DSG2mt/mt mice at these later time points (Fig. 7). Furthermore, cardiac β-myosin heavy chain (βMHC) mRNA, a marker of the fetal gene program , was found to be significantly upregulated in DSG2mt/mt mice at 8 weeks (0.18 ± 0.04 vs. 1.58 ± 0.52 arbitrary units; P = 0.0555) and 13 weeks (1.45 ± 0.06 vs. 2.51 ± 0.29 arbitrary units; P = 0.0095). In contrast, cardiac RGS2 mRNA expression did not differ or tended to be lower in mutants at the studied ages (Fig. 7). mRNA expression of the various markers was not different between wild-type and heterozygous mutant animals at 8 and 13 weeks, which was in line with the absence of changes in morphology and proliferation (e.g., Fig. 5).
DSG2mt/mt mice, which lack major parts of the adhesive extracellular domains EC1 and EC2, develop cardiac fibrosis, ventricular dilation, arrhythmia and cardiac insufficiency. In other Dsg2-producing tissues such as intestinal mucosa, liver and endometrium no overt pathological alterations were observed. Yet, the majority of mutants do not survive until birth and we are presently trying to identify the critical developmental stages. Of note, all mutants that were born displayed normal heart morphology and all of them developed the described cardiac phenotype. These DSG2mt/mt mice expand currently available murine models of desmosome-related dilative cardiomyopathy (DCM) and ARVC [18, 33, 47, 65]. They allow, for the first time, to study the consequences of a mutated endogenous desmosomal protein. The deleted parts of the EC1 and EC2 domains are also affected by mutations in human ARVC and DCM patients [2, 4, 48, 51]. Of note, the pathogenesis of the human diseases is considered to occur in most instances in a dominant-negative fashion [2, 24]. While this may also be the case in the mutant mice, e.g., by altered interactions of the mutant Dsg2, the reduced expression of mutant Dsg2 and its correct targeting to desmosomes argue in favor of a hypomorphic, i.e., loss of function phenotype.
The rationale for deleting a segment of the EC1–EC2 domains was based on the alleged importance of these regions for efficient desmosomal delivery and adhesive interactions. The deleted region encompasses the single N-glycosylation site and one of the four calcium-binding pockets. Although glycosylation may affect trafficking of desmogleins , it appears to be of no or only minor importance in our animal model, since the mutant Dsg2 is delivered to desmosomes. The same is also observed in cDNA-transfected cell lines, in which we do not find a significantly elevated ER- or Golgi localization of the mutant Dsg2 in comparison to its wild-type counterpart . Although the conserved aminoterminal “tryptophane 2”, which has been implicated in cis and trans interactions in other cadherins, is still present in the mutant Dsg2, its hydrophobic binding pocket is deleted (cf. ). Other amino acids that are probably involved in cadherin–cadherin interactions are also missing [7, 52, 59]. Most importantly, the overall shortening of the extracellular domains in combination with the loss of calcium binding, which is presumably involved in stabilization of the EC domains [42, 44, 52], is expected to lead to reduced EC domain overlap for homophilic cis and trans interactions [7, 10, 28, 52, 59]. Heterophilic interactions with desmocollin 2 may be affected in a similar way. Taken together, decreased transcellular flexibility and adhesion would be expected in tissues producing the mutant Dsg2. Further in vivo evidence for the importance of the EC1/EC2 domains can be derived from observations in human diseases. Thus, the deleted Dsg2 domains contain epitopes corresponding to regions of epidermal desmoglein 3 that are targeted by pemphigus autoimmune antibodies, which disrupt epidermal cell–cell adhesion [25, 62]. The reduced adhesiveness of Dsg2mt and the decreased desmosomal localization of Dsg2mt may therefore weaken intercalated discs and lead to impaired force transmission making mutant hearts more susceptible to microtraumata. Dsgs, however, also fulfill functions in signaling. Thus, blockade of specific Dsg1 and 3 epitopes, which correspond to the region deleted in our Dsg2 mutants, by pemphigus autoantibodies affects signaling pathways involving p38, RhoA, protein kinase Cα and cdk-2 in skin [6, 11, 36, 63]. Furthermore, loss of desmosomal cadherins and associated proteins interferes with wnt/β-catenin signaling [18, 28], which has been implicated in DCM . Further investigations are needed to clarify the exact mechanism(s).
The DSG2mt/mt mice helped to identify details of cardiac disease before macroscopic and microscopic alterations became apparent. Thus, c-myc and CTGF mRNAs were already increased in 2-week-old mutant mice with seemingly normal heart muscle indicating early compensatory reactions of the heart to DSG2 gene mutation. In accordance, it has been reported that c-myc and CTGF are involved in the regulation of the cardiac hypertrophic response and cardiac remodeling upon cardiac stress [16, 29, 35, 45]. In the presence of overt cardiomyocyte death and fibrosis, GDF15 mRNA expression was also found to be significantly elevated in 2-week-old mutants. GDF15 is a reliable marker of heart failure in humans  and exerts antihypertrophic and cardioprotective effects [32, 64]. This observation can be taken as an indication of a later step of disease progression, whereby altered cardiac wall compliance is compensated for in addition to Dsg2 malfunction. Disease progression and cardiac response were reflected by further increase of the cardiac stress marker mRNAs and the onset of mRNA synthesis of the natriuretic peptides ANF and BNF in 8-week mutant animals, all of which showed fibrosis and cardiomyocyte death. ANF and BNF effect diuresis and vasodilation and exert an antifibrotic activity in the heart [41, 43].
The fibrotic response in the DSG2mt/mt mice could be subdivided into interstitial fibrosis affecting the entire heart muscle and focal fibrosis or scarring. Interstitial cell proliferation subsides in wild-type animals at 8 weeks, when the heart reaches maturity . In mutant mice, however, the decline of interstitial cell proliferation rate was postponed to the age of 13 weeks. The prolonged interstitial cell proliferation may be a compensation for the compromised Dsg2 function in force transmission at the intercalated discs. In contrast to interstitial fibrosis, fibrotic foci were characterized early on by Kossa-positive cardiomyocytes indicative of necrotic calcinosis . These foci appear in bursts during disease progression and were observed as early as 2 weeks after birth and also later on. Interestingly, similar cardiac necrosis was also described in mice overexpressing a mutant human DSG2 gene . These fibrotic bursts may be induced by local injuries of the heart muscle, which result initially in local increase of fibroblast proliferation and are coupled to increased apoptosis as evidenced by elevated cleaved caspase 3-staining and abundant cardiomyocyte necrosis. Our findings further suggest that these active replacement processes were terminated by the formation of inactive scar tissue that was abundant in the older mutants. It will be interesting to find out whether the peculiar patterns of fibrosis can be correlated with the complex cardiac fiber orientation and force distribution fields . The pronounced histomorphological alterations in cardiac architecture lead to increased wall stiffness and are responsible for the right ventricular dilation that was observed at autopsy and the pathological echocardiographic findings of left ventricular dilation in combination with systolic and diastolic dysfunction. Biventricular dilation probably plays a major part in triggering arrhythmias leading to premature death.
Taken together, the DSG2mt/mt mice revealed major features of desmosome-related cardiomyopathies. Further assessment will clarify steps of disease initiation and progression and will help to develop and test therapeutic strategies for treatment of human DCM and ARVC.
Below is the link to the electronic supplementary material.Click here for additional data file (395_2011_175_MOESM1_ESM.pdf)
Click here for additional data file (395_2011_175_MOESM2_ESM.pdf)
The authors C.A. Krusche and B. Holthöfer contributed equally.
We wish to thank Drs. David Garrod, Gale Martin and Francis Stewart for various reagents and Diana Seelis-Schmidt, Ursula Disque-Kaiser, Ursula Kirmair and Vanessa Creutz for expert technical assistance. We also want to thank Svetlana Ohngemach for help with blastocyst injections and embryo transfers, Rosario Heck for help with embryonal stem cell work, the animal technicians of the Medical Centers in Mainz and Aachen for their excellent animal care and Dr. Andreas Herrler for help during intermediate stages of the project. The work was supported by the Deutsche Krebshilfe and the German Research Council (LE 566/11-1 to REL, Mc1821/3-1 to MWM).
|1..||Al-Amoudi A,Diez DC,Betts MJ,Frangakis AS. The molecular architecture of cadherins in native epidermal desmosomesNatureYear: 200745083283710.1038/nature0599418064004|
|2..||Awad MM,Calkins H,Judge DP. Mechanisms of disease: molecular genetics of arrhythmogenic right ventricular dysplasia/cardiomyopathyNat Clin Pract Cardiovasc MedYear: 2008525826710.1038/ncpcardio118218382419|
|3..||Basso C,Corrado D,Marcus FI,Nava A,Thiene G. Arrhythmogenic right ventricular cardiomyopathyLancetYear: 20093731289130010.1016/S0140-6736(09)60256-719362677|
|4..||Basso C,Czarnowska E,Della Barbera M,Bauce B,Beffagna G,Wlodarska EK,Pilichou K,Ramondo A,Lorenzon A,Wozniek O,Corrado D,Daliento L,Danieli GA,Valente M,Nava A,Thiene G,Rampazzo A. Ultrastructural evidence of intercalated disc remodelling in arrhythmogenic right ventricular cardiomyopathy: an electron microscopy investigation on endomyocardial biopsiesEur Heart JYear: 2006271847185410.1093/eurheartj/ehl09516774985|
|5..||Beffagna G,Occhi G,Nava A,Vitiello L,Ditadi A,Basso C,Bauce B,Carraro G,Thiene G,Towbin JA,Danieli GA,Rampazzo A. Regulatory mutations in transforming growth factor-beta3 gene cause arrhythmogenic right ventricular cardiomyopathy type 1Cardiovasc ResYear: 20056536637310.1016/j.cardiores.2004.10.00515639475|
|6..||Berkowitz P,Chua M,Liu Z,Diaz LA,Rubenstein DS. Autoantibodies in the autoimmune disease pemphigus foliaceus induce blistering via p38 mitogen-activated protein kinase-dependent signaling in the skinAm J PatholYear: 20081731628163610.2353/ajpath.2008.08039118988808|
|7..||Boggon TJ,Murray J,Chappuis-Flament S,Wong E,Gumbiner BM,Shapiro L. C-cadherin ectodomain structure and implications for cell adhesion mechanismsScienceYear: 20022961308131310.1126/science.107155911964443|
|8..||Buchholz F,Angrand PO,Stewart AF. Improved properties of FLP recombinase evolved by cycling mutagenesisNat BiotechnolYear: 19981665766210.1038/nbt0798-6579661200|
|9..||Calkins H,Marcus F. Arrhythmogenic right ventricular cardiomyopathy/dysplasia: an updateCurr Cardiol RepYear: 20081036737510.1007/s11886-008-0059-418715533|
|10..||Chappuis-Flament S,Wong E,Hicks LD,Kay CM,Gumbiner BM. Multiple cadherin extracellular repeats mediate homophilic binding and adhesionJ Cell BiolYear: 200115423124310.1083/jcb.20010314311449003|
|11..||Chernyavsky AI,Arredondo J,Kitajima Y,Sato-Nagai M,Grando SA. Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigensJ Biol ChemYear: 2007282138041381210.1074/jbc.M61136520017344213|
|12..||Chitaev NA,Averbakh AZ,Troyanovsky RB,Troyanovsky SM. Molecular organization of the desmoglein–plakoglobin complexJ Cell SciYear: 1998111Pt 14194119499645942|
|13..||Chitaev NA,Troyanovsky SM. Direct Ca2+-dependent heterophilic interaction between desmosomal cadherins, desmoglein and desmocollin, contributes to cell–cell adhesionJ Cell BiolYear: 199713819320110.1083/jcb.138.1.1939214392|
|14..||Dorn GW 2nd,Robbins J,Sugden PH. Phenotyping hypertrophy: eschew obfuscationCirc ResYear: 2003921171117510.1161/01.RES.0000077012.11088.BC12805233|
|15..||Eshkind L,Tian Q,Schmidt A,Franke WW,Windoffer R,Leube RE. Loss of desmoglein 2 suggests essential functions for early embryonic development and proliferation of embryonal stem cellsEur J Cell BiolYear: 20028159259810.1078/0171-9335-0027812494996|
|16..||Frank D,Kuhn C,Brors B,Hanselmann C,Ludde M,Katus HA,Frey N. Gene expression pattern in biomechanically stretched cardiomyocytes: evidence for a stretch-specific gene programHypertensionYear: 20085130931810.1161/HYPERTENSIONAHA.107.09804618158353|
|17..||Fressart V,Duthoit G,Donal E,Probst V,Deharo JC,Chevalier P,Klug D,Dubourg O,Delacretaz E,Cosnay P,Scanu P,Extramiana F,Keller D,Hidden-Lucet F,Simon F,Bessirard V,Roux-Buisson N,Hebert JL,Azarine A,Casset-Senon D,Rouzet F,Lecarpentier Y,Fontaine G,Coirault C,Frank R,Hainque B,Charron P. Desmosomal gene analysis in arrhythmogenic right ventricular dysplasia/cardiomyopathy: spectrum of mutations and clinical impact in practiceEuropaceYear: 20101286186810.1093/europace/euq10420400443|
|18..||Garcia-Gras E,Lombardi R,Giocondo MJ,Willerson JT,Schneider MD,Khoury DS,Marian AJ. Suppression of canonical Wnt/beta-catenin signaling by nuclear plakoglobin recapitulates phenotype of arrhythmogenic right ventricular cardiomyopathyJ Clin InvestYear: 20061162012202110.1172/JCI2775116823493|
|19..||Garrod D,Chidgey M. Desmosome structure, composition and functionBiochim Biophys ActaYear: 2008177857258710.1016/j.bbamem.2007.07.01417854763|
|20..||Gerull B,Heuser A,Wichter T,Paul M,Basson CT,McDermott DA,Lerman BB,Markowitz SM,Ellinor PT,MacRae CA,Peters S,Grossmann KS,Drenckhahn J,Michely B,Sasse-Klaassen S,Birchmeier W,Dietz R,Breithardt G,Schulze-Bahr E,Thierfelder L. Mutations in the desmosomal protein plakophilin-2 are common in arrhythmogenic right ventricular cardiomyopathyNat GenetYear: 2004361162116410.1038/ng146115489853|
|21..||Green KJ,Simpson CL. Desmosomes: new perspectives on a classicJ Invest DermatolYear: 20071272499251510.1038/sj.jid.570101517934502|
|22..||Harrell MD,Harbi S,Hoffman JF,Zavadil J,Coetzee WA. Large-scale analysis of ion channel gene expression in the mouse heart during perinatal developmentPhysiol GenomicsYear: 20072827328316985003|
|23..||He W,Cowin P,Stokes DL. Untangling desmosomal knots with electron tomographyScienceYear: 200330210911310.1126/science.108695714526082|
|24..||Herren T,Gerber PA,Duru F. Arrhythmogenic right ventricular cardiomyopathy/dysplasia: a not so rare “disease of the desmosome” with multiple clinical presentationsClin Res CardiolYear: 20099814115810.1007/s00392-009-0751-419205777|
|25..||Heupel WM,Zillikens D,Drenckhahn D,Waschke J. Pemphigus vulgaris IgG directly inhibit desmoglein 3-mediated transinteractionJ ImmunolYear: 20081811825183418641320|
|26..||Hirschy A,Croquelois A,Perriard E,Schoenauer R,Agarkova I,Hoerstrup SP,Taketo MM,Pedrazzini T,Perriard JC,Ehler E. Stabilised beta-catenin in postnatal ventricular myocardium leads to dilated cardiomyopathy and premature deathBasic Res CardiolYear: 201010559760810.1007/s00395-010-0101-820376467|
|27..||Holthofer B. Konditionale Mutagenese von Desmoglein 2 in der MausYear: 2007MainzInstitute of Anatomy. Johannes Gutenberg-University1208|
|28..||Holthofer B,Windoffer R,Troyanovsky S,Leube RE. Structure and function of desmosomesInt Rev CytolYear: 20072646516310.1016/S0074-7696(07)64003-017964922|
|29..||Ihm SH,Chang K,Kim HY,Baek SH,Youn HJ,Seung KB,Kim JH. Peroxisome proliferator-activated receptor-gamma activation attenuates cardiac fibrosis in type 2 diabetic rats: the effect of rosiglitazone on myocardial expression of receptor for advanced glycation end products and of connective tissue growth factorBasic Res CardiolYear: 201010539940710.1007/s00395-009-0071-x19902320|
|30..||Kelly D,Mackenzie L,Hunter P,Smaill B,Saint DA. Gene expression of stretch-activated channels and mechanoelectric feedback in the heartClin Exp Pharmacol PhysiolYear: 20063364264810.1111/j.1440-1681.2006.04392.x16789934|
|31..||Kempf T,Haehling S,Peter T,Allhoff T,Cicoira M,Doehner W,Ponikowski P,Filippatos GS,Rozentryt P,Drexler H,Anker SD,Wollert KC. Prognostic utility of growth differentiation factor-15 in patients with chronic heart failureJ Am Coll CardiolYear: 2007501054106010.1016/j.jacc.2007.04.09117825714|
|32..||Khan SQ,Ng K,Dhillon O,Kelly D,Quinn P,Squire IB,Davies JE,Ng LL. Growth differentiation factor-15 as a prognostic marker in patients with acute myocardial infarctionEur Heart JYear: 2009301057106510.1093/eurheartj/ehn60019168526|
|33..||Kirchhof P,Fabritz L,Zwiener M,Witt H,Schafers M,Zellerhoff S,Paul M,Athai T,Hiller KH,Baba HA,Breithardt G,Ruiz P,Wichter T,Levkau B. Age- and training-dependent development of arrhythmogenic right ventricular cardiomyopathy in heterozygous plakoglobin-deficient miceCirculationYear: 20061141799180610.1161/CIRCULATIONAHA.106.62450217030684|
|34..||Korff S,Riechert N,Schoensiegel F,Weichenhan D,Autschbach F,Katus HA,Ivandic BT. Calcification of myocardial necrosis is common in miceVirchows ArchYear: 200644863063810.1007/s00428-005-0071-716211391|
|35..||Kroumpouzou E,Gomatos IP,Kataki A,Karayannis M,Dangas GD,Toutouzas P. Common pathways for primary hypertrophic and dilated cardiomyopathyHybrid HybridomicsYear: 200322414510.1089/15368590332153807112713689|
|36..||Lanza A,Cirillo N,Rossiello R,Rienzo M,Cutillo L,Casamassimi A,Nigris F,Schiano C,Rossiello L,Femiano F,Gombos F,Napoli C. Evidence of key role of Cdk2 overexpression in pemphigus vulgarisJ Biol ChemYear: 20082838736874510.1074/jbc.M70218620018199752|
|37..||Leu M,Ehler E,Perriard JC. Characterisation of postnatal growth of the murine heartAnat Embryol (Berl)Year: 200120421722410.1007/s00429010020611681801|
|38..||Lewandoski M,Martin GR. Cre-mediated chromosome loss in miceNat GenetYear: 19971722322510.1038/ng1097-2239326948|
|39..||Merner ND,Hodgkinson KA,Haywood AF,Connors S,French VM,Drenckhahn JD,Kupprion C,Ramadanova K,Thierfelder L,McKenna W,Gallagher B,Morris-Larkin L,Bassett AS,Parfrey PS,Young TL. Arrhythmogenic right ventricular cardiomyopathy type 5 is a fully penetrant, lethal arrhythmic disorder caused by a missense mutation in the TMEM43 geneAm J Hum GenetYear: 20088280982110.1016/j.ajhg.2008.01.01018313022|
|40..||Merx MW,Schafer C,Westenfeld R,Brandenburg V,Hidajat S,Weber C,Ketteler M,Jahnen-Dechent W. Myocardial stiffness, cardiac remodeling, and diastolic dysfunction in calcification-prone fetuin-A-deficient miceJ Am Soc NephrolYear: 2005163357336410.1681/ASN.200504036516177000|
|41..||Molkentin JD. A friend within the heart: natriuretic peptide receptor signalingJ Clin InvestYear: 20031111275127712727915|
|42..||Nagar B,Overduin M,Ikura M,Rini JM. Structural basis of calcium-induced E-cadherin rigidification and dimerizationNatureYear: 199638036036410.1038/380360a08598933|
|43..||Nishikimi T,Maeda N,Matsuoka H. The role of natriuretic peptides in cardioprotectionCardiovasc ResYear: 20066931832810.1016/j.cardiores.2005.10.00116289003|
|44..||Overduin M,Harvey TS,Bagby S,Tong KI,Yau P,Takeichi M,Ikura M. Solution structure of the epithelial cadherin domain responsible for selective cell adhesionScienceYear: 199526738638910.1126/science.78249377824937|
|45..||Panek AN,Posch MG,Alenina N,Ghadge SK,Erdmann B,Popova E,Perrot A,Geier C,Dietz R,Morano I,Bader M,Ozcelik C. Connective tissue growth factor overexpression in cardiomyocytes promotes cardiac hypertrophy and protection against pressure overloadPLoS OneYear: 20094e674310.1371/journal.pone.000674319707545|
|46..||Pasdar M,Nelson WJ. Regulation of desmosome assembly in epithelial cells: kinetics of synthesis, transport, and stabilization of desmoglein I, a major protein of the membrane core domainJ Cell BiolYear: 198910916317710.1083/jcb.109.1.1632501314|
|47..||Pilichou K,Remme CA,Basso C,Campian ME,Rizzo S,Barnett P,Scicluna BP,Bauce B,Hoff MJ,Bakker JM,Tan HL,Valente M,Nava A,Wilde AA,Moorman AF,Thiene G,Bezzina CR. Myocyte necrosis underlies progressive myocardial dystrophy in mouse dsg2-related arrhythmogenic right ventricular cardiomyopathyJ Exp MedYear: 20092061787180210.1084/jem.2009064119635863|
|48..||Posch MG,Posch MJ,Geier C,Erdmann B,Mueller W,Richter A,Ruppert V,Pankuweit S,Maisch B,Perrot A,Buttgereit J,Dietz R,Haverkamp W,Ozcelik C. A missense variant in desmoglein-2 predisposes to dilated cardiomyopathyMol Genet MetabYear: 200895748010.1016/j.ymgme.2008.06.00518678517|
|49..||Rodriguez CI,Buchholz F,Galloway J,Sequerra R,Kasper J,Ayala R,Stewart AF,Dymecki SM. High-efficiency deleter mice show that FLPe is an alternative to Cre-loxPNat GenetYear: 20002513914010.1038/7597310835623|
|50..||Schlegel N,Meir M,Heupel WM,Holthofer B,Leube RE,Waschke J. Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrityAm J Physiol Gastrointest Liver PhysiolYear: 2010298G774G78310.1152/ajpgi.00239.200920224006|
|51..||Sen-Chowdhry S,Syrris P,McKenna WJ. Role of genetic analysis in the management of patients with arrhythmogenic right ventricular dysplasia/cardiomyopathyJ Am Coll CardiolYear: 2007501813182110.1016/j.jacc.2007.08.00817980246|
|52..||Shapiro L,Fannon AM,Kwong PD,Thompson A,Lehmann MS,Grubel G,Legrand JF,Als-Nielsen J,Colman DR,Hendrickson WA. Structural basis of cell–cell adhesion by cadherinsNatureYear: 199537432733710.1038/374327a07885471|
|53..||Shimizu A,Ishiko A,Ota T,Saito H,Oka H,Tsunoda K,Amagai M,Nishikawa T. In vivo ultrastructural localization of the desmoglein 3 adhesive interface to the desmosome mid-lineJ Invest DermatolYear: 200512498498910.1111/j.0022-202X.2005.23706.x15854040|
|54..||Sugden PH,Clerk A. Cellular mechanisms of cardiac hypertrophyJ Mol MedYear: 19987672574610.1007/s0010900502759826118|
|55..||Syed SE,Trinnaman B,Martin S,Major S,Hutchinson J,Magee AI. Molecular interactions between desmosomal cadherinsBiochem JYear: 200236231732710.1042/0264-6021:362031711853539|
|56..||Szabo P,Mann JR. Expression and methylation of imprinted genes during in vitro differentiation of mouse parthenogenetic and androgenetic embryonic stem cell linesDevelopmentYear: 1994120165116608050371|
|57..||Thiene G,Corrado D,Basso C. Arrhythmogenic right ventricular cardiomyopathy/dysplasiaOrphanet J Rare DisYear: 200724510.1186/1750-1172-2-4518001465|
|58..||Tiso N,Stephan DA,Nava A,Bagattin A,Devaney JM,Stanchi F,Larderet G,Brahmbhatt B,Brown K,Bauce B,Muriago M,Basso C,Thiene G,Danieli GA,Rampazzo A. Identification of mutations in the cardiac ryanodine receptor gene in families affected with arrhythmogenic right ventricular cardiomyopathy type 2 (ARVD2)Hum Mol GenetYear: 20011018919410.1093/hmg/10.3.18911159936|
|59..||Troyanovsky S. Cadherin dimers in cell–cell adhesionEur J Cell BiolYear: 20058422523310.1016/j.ejcb.2004.12.00915819403|
|60..||Troyanovsky SM. Mechanism of cell–cell adhesion complex assemblyCurr Opin Cell BiolYear: 19991156156610.1016/S0955-0674(99)00021-610508653|
|61..||Tsatsopoulou AA,Protonotarios NI,McKenna WJ. Arrhythmogenic right ventricular dysplasia, a cell adhesion cardiomyopathy: insights into disease pathogenesis from preliminary genotype–phenotype assessmentHeartYear: 2006921720172310.1136/hrt.2005.08167916698823|
|62..||Tsunoda K,Ota T,Aoki M,Yamada T,Nagai T,Nakagawa T,Koyasu S,Nishikawa T,Amagai M. Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3J ImmunolYear: 20031702170217812574390|
|63..||Waschke J,Spindler V,Bruggeman P,Zillikens D,Schmidt G,Drenckhahn D. Inhibition of Rho A activity causes pemphigus skin blisteringJ Cell BiolYear: 200617572172710.1083/jcb.20060512517130286|
|64..||Xu J,Kimball TR,Lorenz JN,Brown DA,Bauskin AR,Klevitsky R,Hewett TE,Breit SN,Molkentin JD. GDF15/MIC-1 functions as a protective and antihypertrophic factor released from the myocardium in association with SMAD protein activationCirc ResYear: 20069834235010.1161/01.RES.0000202804.84885.d016397142|
|65..||Yang Z,Bowles NE,Scherer SE,Taylor MD,Kearney DL,Ge S,Nadvoretskiy VV,DeFreitas G,Carabello B,Brandon LI,Godsel LM,Green KJ,Saffitz JE,Li H,Danieli GA,Calkins H,Marcus F,Towbin JA. Desmosomal dysfunction due to mutations in desmoplakin causes arrhythmogenic right ventricular dysplasia/cardiomyopathyCirc ResYear: 20069964665510.1161/01.RES.0000241482.19382.c616917092|
|66..||Zhang Y,Takagawa J,Sievers RE,Khan MF,Viswanathan MN,Springer ML,Foster E,Yeghiazarians Y. Validation of the wall motion score and myocardial performance indexes as novel techniques to assess cardiac function in mice after myocardial infarctionAm J Physiol Heart Circ PhysiolYear: 2007292H1187H119210.1152/ajpheart.00895.200617028161|
|ARVC||Arrhythmogenic right ventricular cardiomyopathy|
|DSG2||Gene encoding Dsg2|
|Dsg2||Desmoglein 2 polypeptide|
List of synthetic oligonucleotides
Clinical measurements reveal deficiencies in right and left ventricular functions of DSG2mt/mt mutant mice
|Body weight (g)||32.16 ± 2.06||33.89 ± 2.69|
|Heart weight (mg)||174.16 ± 16.34||221.66 ± 13.19***|
|Heart weight/body weight||5.41 ± 0.22||6.59 ± 0.69**|
|Heart length (apex basis, mm)||4.76 ± 0.2||5.55 ± 0.48**|
|Heart rate (beats/min)||502.75 ± 67.87||456.83 ± 38.45|
|Left ventricle (LV) mass (mg)||115.48 ± 24.49||186.85 ± 80.66*|
|LV mass/body mass (mg/g)||3.57 ± 0.65||5.5 ± 2.26*|
|Interventricular septum in diastole (mm)||0.71 ± 0.07||0.79 ± 0.1|
|LV inner diameter in diastole (mm)||4.28 ± 0.49||5.23 ± 1.15 (P = 0.05)|
|LV posterior wall in diastole (mm)||0.73 ± 0.18||0.77 ± 0.12|
|Interventricular septum in systole (mm)||1.01 ± 0.13||0.90 ± 0.12|
|LV inner diameter in systole (mm)||2.68 ± 0.48||4.39 ± 1.23**|
|LV posterior wall in systole (mm)||1.12 ± 0.34||0.98 ± 0.18|
|Fractional shortening (%)||37.77 ± 4.59||16.98 ± 5.19***|
|Ejection fraction (%)||67.00 ± 5.18||34.43 ± 11.28***|
|Cardiac output (ml/min)||24.73 ± 3.57||17.43 ± 2.79**|
|Stroke volume (μl)||50.81 ± 7.51||37.97 ± 6.67**|
|Fractional area change (%)||52.46 ± 8.48||23.82 ± 8.83***|
|End-diastolic volume (μl)||76.33 ± 14.41||121.82 ± 49.70*|
|End-systolic volume (μl)||26.61 ± 8.89||83.85 ± 48.17**|
|End-diastolic volume index (μl/g)||2.37 ± 0.44||3.61 ± 1.47*|
|End-systolic volume index (μl/g)||0.82 ± 0.26||2.49 ± 1.4***|
|Doppler wave form|
|Mitral valve E-wave peak (cm/s)||785.64 ± 105.67||739.62 ± 153.82 (P = 0,05)|
|Mitral valve A-wave peak (cm/s)||557.31 ± 144.52||244.25 ± 135.75**|
|Mitral valve E/A||1.46 ± 0.27||3.77 ± 2.01***|
|Left ventricular developed pressure (LVDP)baseline||104.00 ± 3.81||92.71 ± 5.57**|
|LVDPnorepinephrine||120.00 ± 7.95||105.99 ± 9.66*|
|+dP/dtmax baseline||13,568.75 ± 865.77||9,522.14 ± 1,778.36***|
|+dP/dtmax norepinephrine||16,959.37 ± 1,311.36||11,916.14 ± 2,293.64***|
|−dP/dtmin baseline||−9,147.13 ± 1,209.85||−6,376.58 ± 927.54**|
|−dP/dtmin norepinephrine||−9,958.25 ± 1,293.03||−7,450.29 ± 1,218.84**|
|Relaxation indexbaseline||−87.92 ± 10.81||−68.50 ± 6.80**|
|Relaxation indexnorepinehrine||−83.09 ± 10.30||−70.09 ± 8.64*|
M-mode and B-mode measurements indicate left ventricular dilatation in the mutant mice accompanied by decreased systolic function (echocardiography and invasive hemodynamics). Note the decreased left ventricular compliance and impaired left atrial contractility. Late diastolic velocity was reduced with consequently increased E/A; (wild-type DSG2wt/wt vs. DSG2mt/mt mutants *P < 0.05, **P < 0.01, ***P < 0.001; mean ± SD)
Cardiac cytokine mRNA expression in relation to fibrosis in 2-week-old DSG2-mutant mice
|Cytokine mRNA||wt/wt (n = 3)||mt/wt (n = 4)||mt/mt − fibr. (n = 4)||mt/mt + fibr. (n = 3)||ANOVA|
|ANF||0.26 ± 0.14||1.12 ± 0.63||0.64 ± 0.25||1.82 ± 0.27||0.1371|
|BNF||0.34 ± 0.05||0.87 ± 0.15||1.13 ± 0.32||1.59 ± 0.54||0.1196|
|c-myc||0.40 ± 0.09||0.47 ± 0.04||1.14 ± 0.48||2.04 ± 0.81||0.1006|
|CTGF||0.12 ± 0.04||0.18 ± 0.01||0.34 ± 0.07||0.53 ± 0.15*||0.0191|
|GDF15||0.18 ± 0.03||0.31 ± 0.09||0.82 ± 0.30||3.83 ± 1.42*,**,+||0.0083|
|RGS2||0.69 ± 0.10||0.77 ± 0.24||0.85 ± 0.15||0.76 ± 0.07||0.9015|
The results of cytokine RT-PCR analyses in hearts of wild-type (wt/wt), heterozygous (mt/wt) and homozygous DSG2-mutant mice (mt/mt) are summarized. The homozygous mutant mice are further subclassified into those with severe cardiac fibrosis (mt/mt + fibr.) and those without visible cardiac fibrosis (mt/mt − fibr.). Data are given as mean ± SEM. Note the significantly elevated GDF15 mRNA in the homozygous mutant animals with fibrotic foci
* wt/wt versus mt/mt + fibr. P < 0.05
** mt/wt versus mt/mt + fibr. P < 0.05
+mt/mt − fibr. versus mt/mt + fibr. P < 0.05
Keywords: Keywords Desmoglein 2, Desmosome, Mouse model, Cardiomyopathy, ARVC.
Previous Document: Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North Am...
Next Document: Efficacy of zinc in reducing hyperbilirubinemia among at-risk neonates: a randomized, double-blind, ...