Document Detail


Designs and concept reliance of a fully automated high-content screening platform.
MedLine Citation:
PMID:  22797489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-content screening (HCS) is becoming an accepted platform in academic and industry screening labs and does require slightly different logistics for execution. To automate our stand-alone HCS microscopes, namely, an alpha IN Cell Analyzer 3000 (INCA3000), originally a Praelux unit hooked to a Hudson Plate Crane with a maximum capacity of 50 plates per run, and the IN Cell Analyzer 2000 (INCA2000), in which up to 320 plates could be fed per run using the Thermo Fisher Scientific Orbitor, we opted for a 4 m linear track system harboring both microscopes, plate washer, bulk dispensers, and a high-capacity incubator allowing us to perform both live and fixed cell-based assays while accessing both microscopes on deck. Considerations in design were given to the integration of the alpha INCA3000, a new gripper concept to access the onboard nest, and peripheral locations on deck to ensure a self-reliant system capable of achieving higher throughput. The resulting system, referred to as Hestia, has been fully operational since the new year, has an onboard capacity of 504 plates, and harbors the only fully automated alpha INCA3000 unit in the world.
Authors:
Constantin Radu; Hosna Sana Adrar; Ab Alamir; Ian Hatherley; Trung Trinh; Hakim Djaballah
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-12
Journal Detail:
Title:  Journal of laboratory automation     Volume:  17     ISSN:  2211-0682     ISO Abbreviation:  J Lab Autom     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-21     Completed Date:  2013-01-30     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101558509     Medline TA:  J Lab Autom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  359-69     Citation Subset:  IM    
Affiliation:
Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Automation, Laboratory / methods*
Cytological Techniques / methods*
Drug Evaluation, Preclinical / methods*
High-Throughput Screening Assays*
Humans
Microscopy / methods
Grant Support
ID/Acronym/Agency:
5 P30 CA008748-44/CA/NCI NIH HHS; P30 CA008748/CA/NCI NIH HHS
Comments/Corrections

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