Document Detail

Designing and transgenic expression of melanin gene in tobacco trichome and cotton fiber.
MedLine Citation:
PMID:  17006798     Owner:  NLM     Status:  MEDLINE    
In Streptomyces antibioticus, there are two genes TYRA and ORF438 required for the melanin biogenesis. To investigate whether expression of these two genes in cotton can change cotton fiber colour, we modified the TYRA and ORF438 genes to make their codon usage closer to the codon preference of cotton fiber genes. The resulting versions of these two genes were referred to as DTYRA and DORF438, respectively. Vacuolar targeting signals were also added to their ends. Under the cotton fiber specific LTP3 promoter, DORF438 and DTYRA were first transformed into model plant tobacco (Nicotiana tabacum). Molecular analyses showed that both the DORF438 and DTYRA genes were successfully expressed in transgenic plants, and the melanin deposition was observed in the trichomes of transgenic tobacco. Excitedly, when the same DORF438 and DTYRA expression cassettes were transformed into cotton (Gossypium hirsutum L.) by pollen tube pathway, the colour of cotton fiber changed from white to brown. Molecular analyses confirmed that both genes were transformed into cotton and expressed successfully. All these results indicate that the synthesized DOFR438 and DTYRA genes can work well in tobacco and cotton. Our study may provide a potential method for modifying the colour of cotton fiber.
X Xu; M Wu; Q Zhao; R Li; J Chen; G Ao; J Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-28
Journal Detail:
Title:  Plant biology (Stuttgart, Germany)     Volume:  9     ISSN:  1435-8603     ISO Abbreviation:  Plant Biol (Stuttg)     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-17     Completed Date:  2007-04-03     Revised Date:  2008-01-07    
Medline Journal Info:
Nlm Unique ID:  101148926     Medline TA:  Plant Biol (Stuttg)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  41-8     Citation Subset:  IM    
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Yuanmingyuan West Road No. 2, Haidian District, Beijing 100094, PR China.
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / genetics*,  metabolism
Base Sequence
Gossypium / anatomy & histology,  genetics*
Melanins / genetics*,  metabolism
Molecular Sequence Data
Plants, Genetically Modified / anatomy & histology,  metabolism
Protein Engineering
Protein Transport
Tobacco / genetics*
Reg. No./Substance:
0/Bacterial Proteins; 0/Melanins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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