| Designing polyHEMA substrates that mimic the viscoelastic response of soft tissue. | |
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MedLine Citation:
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PMID: 21496821 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matching the mechanical properties of a biomaterial to soft tissue is often overlooked despite the fact that it is well known that cells respond to and are capable of changing their mechanical environment. In this paper, we used NaCl and alginate beads as porogens to make a series of micro- and macro-porous pHEMA substrates (poly(2-hydroxyethly methacrylate)) and quantified their mechanical behavior under low-magnitude shear loads over physiologically relevant frequencies. Using a stress-controlled rheometer, we performed isothermal (37°C) frequency response experiments between 0.628 and 75.4rad/s (0.01-12Hz) at 0.1% strain. Both micro- and macro-porous pHEMA substrates were predominately elastic in nature with a narrow range of G' and G″ values that mimicked the response of human skin. The magnitude of the G' and G″ values of the macro-porous substrates were designed to closely match human skin. To determine how cell growth might alter their mechanical properties, pHEMA substrates were functionalized and human skin fibroblasts grown on them for fourteen days. As a result of cell growth, the magnitude of G' and G″ increased at low frequencies while also altering the degree of high frequency dependence, indicating that cellular interactions with the micro-pore infrastructure has a profound effect on the viscoelastic behavior of the substrates. These data could be fit to a mathematical model describing a soft-solid. A quantitative understanding of the mechanical behavior of biomaterials in regimes that are physiologically relevant and how these mechanics may change after implantation may aid in the design of new materials. |
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Authors:
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Brian Holt; Anubhav Tripathi; Jeffrey R Morgan |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-04-14 |
Journal Detail:
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Title: Journal of biomechanics Volume: 44 ISSN: 1873-2380 ISO Abbreviation: J Biomech Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-02 Completed Date: 2011-10-03 Revised Date: 2012-05-21 |
Medline Journal Info:
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Nlm Unique ID: 0157375 Medline TA: J Biomech Country: United States |
Other Details:
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Languages: eng Pagination: 1491-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Molecular Pharmacology, Physiology, and Biotechnology, Box GB-393, Brown University, Providence, RI 02912, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Elasticity Fibroblasts / metabolism Humans Kinetics Materials Testing* Polyhydroxyethyl Methacrylate / chemistry* Porosity Rheology / methods Shear Strength Skin / pathology Stress, Mechanical Surface Properties Temperature Viscosity |
| Grant Support | |
ID/Acronym/Agency:
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F31 AR054202-03/AR/NIAMS NIH HHS; F31AR054202/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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25249-16-5/Polyhydroxyethyl Methacrylate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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