Document Detail


Designing nanostructured block copolymer surfaces to control protein adhesion.
MedLine Citation:
PMID:  22509062     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The profile and conformation of proteins that are adsorbed onto a polymeric biomaterial surface have a profound effect on its in vivo performance. Cells and tissue recognize the protein layer rather than directly interact with the surface. The chemistry and morphology of a polymer surface will govern the protein behaviour. So, by controlling the polymer surface, the biocompatibility can be regulated. Nanoscale surface features are known to affect the protein behaviour, and in this overview the nanostructure of self-assembled block copolymers will be harnessed to control protein behaviour. The nanostructure of a block copolymer can be controlled by manipulating the chemistry and arrangement of the blocks. Random, A-B and A-B-A block copolymers composed of methyl methacrylate copolymerized with either acrylic acid or 2-hydroxyethyl methacrylate will be explored. Using atomic force microscopy (AFM), the surface morphology of these block copolymers will be characterized. Further, AFM tips functionalized with proteins will measure the adhesion of that particular protein to polymer surfaces. In this manner, the influence of block copolymer morphology on protein adhesion can be measured. AFM tips functionalized with antibodies to fibronectin will determine how the surfaces will affect the conformation of fibronectin, an important parameter in evaluating surface biocompatibility.
Authors:
Scott R Schricker; Manuel L B Palacio; Bharat Bhushan
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Philosophical transactions. Series A, Mathematical, physical, and engineering sciences     Volume:  370     ISSN:  1364-503X     ISO Abbreviation:  Philos Trans A Math Phys Eng Sci     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-17     Completed Date:  2012-06-06     Revised Date:  2014-04-07    
Medline Journal Info:
Nlm Unique ID:  101133385     Medline TA:  Philos Trans A Math Phys Eng Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2348-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biocompatible Materials
Microscopy, Atomic Force
Nanostructures*
Polymers / chemistry*
Protein Binding
Proteins / chemistry*
Grant Support
ID/Acronym/Agency:
UL1 RR025755/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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