| Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting. | |
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MedLine Citation:
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PMID: 23027051 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Short interfering RNAs (siRNAs) have become valued tools for knocking down specific genes. As such, siRNAs are routinely used to study gene function and are also being explored as therapeutic agents. Traditionally, siRNAs are designed to target one specific gene, but this chapter describes a procedure for designing dual-targeting siRNAs where the two strands in the siRNA duplex are both active and down-regulate different target genes through both siRNA and miRNA-like effects. The procedure can be used to create siRNAs that robustly target pairs of genes. |
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Authors:
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Pål Sætrom |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 942 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 169-77 Citation Subset: IM |
Affiliation:
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Departments of Computer and Information Science and Cancer Research & Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway, pal.satrom@ntnu.no. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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